PXD042494 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparison of LC-MS/MS and real-time PCR assays for the efficient detection of potentially allergenic silkworm |
Description | The silkworm (Bombyx mori) has long been considered a source of food and medicine due to its high nutritional, medicinal, and economic value in East Asia. However, in some sensitive individuals, silkworm consumption can cause allergenic reactions such as vomiting, asthma, and anaphylaxis. Therefore, the development of a reliable method for silkworm detection is required to avoid such allergenic incidents. In this study, two different methods (liquid chromatography combined with mass spectrometry [LC-MS/MS] and real-time polymerase chain reaction [PCR]) were developed to determine an efficient technique for silkworm detection in foods. The developed methods demonstrated high sensitivity in detecting the silkworm in processed foods. Silkworm-spiked model cookies were used to confirm the sensitivity of both LC-MS/MS (0.0005%) and real-time PCR (0.001%). These methods were found to be useful for detecting the silkworm in foods and avoiding allergenic reactions. To the best of our knowledge, this is the first study to compare LC-MS/MS and real-time PCR for silkworm detection in complex processed foods. |
HostingRepository | PRIDE |
AnnounceDate | 2024-05-24 |
AnnouncementXML | Submission_2024-05-24_03:05:42.085.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kyungdo Kim |
SpeciesList | scientific name: Bombyx mori (Silk moth); NCBI TaxID: 7091; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive; Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-05-25 05:19:13 | ID requested | |
⏵ 1 | 2024-05-24 03:05:42 | announced | |
Publication List
Suh SM, Kim K, Yang SM, Lee H, Jun M, Byun J, Lee H, Kim D, Lee D, Cha JE, Kim JS, Kim E, Park ZY, Kim HY, Comparative analysis of LC-MS/MS and real-time PCR assays for efficient detection of potential allergenic silkworm. Food Chem, 445():138761(2024) [pubmed] |
10.1016/j.foodchem.2024.138761; |
Keyword List
submitter keyword: Real-time PCR, Model cookies, Alpha-amlyase, Lipoprotein,Bombyx mori, Silkworm, LC-MS/MS |
Contact List
Zee-Yong Park |
contact affiliation | School of Life Science, Gwangju Institute of Science and Technology, Cheomdangwagiro123, Buk-gu, Gwangju, 61005, Republic of Korea |
contact email | zeeyong@gist.ac.kr |
lab head | |
Kyungdo Kim |
contact affiliation | Gwnagju Institute of Science and Technology |
contact email | rlarudeh10@gm.gist.ac.kr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD042494
- Label: PRIDE project
- Name: Comparison of LC-MS/MS and real-time PCR assays for the efficient detection of potentially allergenic silkworm