<<< Full experiment listing

PXD042487-1

PXD042487 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe CprRS two-component system regulates virulence in Pseudomonas aeruginosa
DescriptionThe ability of Pseudomonas aeruginosa to detect host immune responses and coordinate the production of virulence factors is crucial for its high pathogenicity. However, the regulatory mechanisms underlying this process remain largely unknown. In this study, we extensively characterize the conserved two-component system CprRS in P. aeruginosa and uncover the role of the sensor histidine kinase CprS as a receptor for the human host defense peptide LL-37, thereby modulating bacterial virulence. In the presence of LL-37, CprS functions as a phosphatase, targeting the intracellular activator CprR for phosphorylation at position D53, ultimately leading to CprR activation. By employing quantitative proteomics and transcription analysis, we further elucidate that CprR induction under LL-37 treatment promotes the expression of type III secretion system effectors. This, in turn, impedes the expression of proinflammatory cytokines and significantly increases bacterial cytotoxicity towards macrophages. Notably, the mutation of either cprS or cprR markedly diminishes bacterial survival in both macrophage and insect infection models. Collectively, our study uncovers a novel regulatory mechanism of the two-component system in P. aeruginosa, which enables the bacterium to sense and respond to human innate immune system responses while ensuring a timely balance of virulence genes.
HostingRepositoryiProX
AnnounceDate2023-05-25
AnnouncementXMLSubmission_2023-05-24_19:49:26.502.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSiping Zhang
SpeciesList scientific name: Pseudomonas aeruginosa PA14; NCBI TaxID: 652611;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-05-24 19:49:19ID requested
12023-05-24 19:49:26announced
22023-05-24 19:53:40announced2023-05-25: Update information.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: P. aeruginosa, two-component system, LL-37, type III secretion system
Contact List
Heyong Xing
contact affiliationLanzhou UUniversity
contact emailheyx@lzu.edu.cn
lab head
Siping Zhang
contact affiliationLanzhou UUniversity
contact emailzhangsp2015@lzu.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI