PXD042477-1

PXD042477 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	A Shift Between Mineral and Nonmineral Sources of Iron and Sulfur Causes Proteome-wide Changes in Methanosarcina barkeri
Description	Iron (Fe) and sulfur (S) are required elements for life, and changes in their availability can limit the ecological distribution and function of microorganisms. In anoxic environments, soluble Fe typically exists as ferrous iron (Fe(II)) and S as sulfide (HS-). These species exhibit a strong affinity towards one another, resulting in the formation of sedimentary pyrite (FeS2). Recent, paradigm shifting studies indicate that Fe and S in pyrite can be made bioavailable by methanogens through a reductive dissolution process. However, the impact of the utilization of FeS2, as opposed to canonical Fe and S sources, on the phenotype of cells is not fully understood. Here, shotgun proteomics was utilized to measure changes in the phenotype of Methanosarcina barkeri grown with FeS2, Fe(II)/HS-; or Fe(II)/cysteine. Shotgun proteomics tracked 1019 proteins, with 307 observed to change significantly between growth conditions. Functional characterization and pathway analyses revealed these changes to be systemic, and largely tangential to Fe/S metabolism. As a final step, the proteomics data was viewed with respect to previously collected transcriptomics data to deepen the analysis. Presented here is evidence that M. barkeri adopts distinct phenotypes to exploit specific sources of Fe and S in their environment. This is supported by observed protein abundance changes across broad categories of cellular biology. DNA adjacent metabolism, central carbon metabolism / methanogenesis, metal trafficking, quorum sensing and porphyrin biosynthesis pathways are all features in the phenotypic differentiation.
HostingRepository	MassIVE
AnnounceDate	2023-06-06
AnnouncementXML	Submission_2023-06-06_08:41:37.681.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	HUNTER L FAUSSET
SpeciesList	scientific name: Methanosarcina barkeri; NCBI TaxID: 2208;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-24 09:41:17	ID requested
⏵ 1	2023-06-06 08:41:38	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Methanogen, Iron-Sulfur, Proteomics

submitter keyword: Methanogen, Iron-Sulfur, Proteomics

Contact List

Brian Bothner
contact affiliation	Montana State University
contact email	bbothner@montana.edu
lab head
HUNTER L FAUSSET
contact affiliation	montana state university
contact email	hunterfausset@montana.edu
dataset submitter

Full Dataset Link List

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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000092027/