PXD042447-1

PXD042447 is an original dataset announced via ProteomeXchange.

Title	Prenatal delta-9-tetrahydrocannabinol Exposure is Associated with Altered Fetal Neurodevelopment in Rhesus Macaques
Description	Cannabis use during pregnancy is associated with adverse neurodevelopmental outcomes. However, the underlying mechanisms and effects of cannabis on fetal development are relatively unknown. We determined the impact of chronic delta-9-tetrahydrocannabinol (THC, cannabis’s main psychoactive component) exposure on fetal development in a rhesus macaque model using advanced imaging combined with molecular and tissue studies. Animals were divided into control (n=5) or THC-exposed (n=5) groups, which received a daily THC edible pre-conception and throughout pregnancy. Animals underwent fetal brain T2-weighted MRI at gestational days 85 (G85), G110, G135 and G155 (term is ~G168) to assess brain development. All animals underwent cesarean delivery with fetal cerebral spinal fluid (CSF) and brain collection at G155 for microRNA (miRNA) and histologic analysis. THC exposure did not demonstrate any fetal brain volumetric changes. However, histological findings in THC-exposed brains suggested brain dysregulation that may have long-term developmental implications. We identified two extracellular-vesicle-associated miRNAs (mir-448 and mir-199a-3p) with>2-fold change in CSF with maternal THC use. Amniotic fluid (AF) was collected at the 4 gestational ages (GA) and relative protein abundance changes measured with isobaric labeling quantitative proteomics. Changes in AF were observed across the GAs and in response to THC-treatment.
HostingRepository	PRIDE
AnnounceDate	2025-07-19
AnnouncementXML	Submission_2025-07-18_16:12:26.484.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Phillip Wilmarth
SpeciesList	scientific name: Macaca mulatta (Rhesus macaque); NCBI TaxID: 9544;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-23 16:44:01	ID requested
⏵ 1	2025-07-18 16:12:27	announced

Ryan KS, Karpf JA, Chan CN, Hagen OL, McFarland TJ, Urian JW, Wang X, Boniface ER, Hakar MH, Terrobias JJD, Graham JA, Passmore S, Grant KA, Sullivan EL, Grafe MR, Saugstad JA, Kroenke CD, Lo JO, Prenatal delta-9-tetrahydrocannabinol exposure alters fetal neurodevelopment in rhesus macaques. Sci Rep, 14(1):5808(2024) [pubmed]

10.1038/s41598-024-56386-7;

submitter keyword: neurodevelopment, rhesus macaque, isobaric labeling, pregnancy,cannabis, quantitative proteomics, fetal brain, delta-9-tetrahydrocannabinol

Jamie Lo, MD MCR
contact affiliation	Division of Maternal Fetal Medicine Department of Obstetrics and Gynecology Oregon Health & Science University 3181 SW Sam Jackson Park Road, Mail Code L-458, Portland, OR, 97239, USA
contact email	loj@ohsu.edu
lab head
Phillip Wilmarth
contact affiliation	OHSU
contact email	wilmarth@ohsu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD042447

PRIDE project URI

• PRIDE
  1. PXD042447
     1. Label: PRIDE project
     2. Name: Prenatal delta-9-tetrahydrocannabinol Exposure is Associated with Altered Fetal Neurodevelopment in Rhesus Macaques