PXD042445-1

PXD042445 is an original dataset announced via ProteomeXchange.

Title	Shotgun Proteomics to characterize wastewater proteins
Description	Wastewater has been extensively studied along the years. However, these studies have been focused on the analysis of small molecules. There are no studies about the proteins present in wastewater and let alone an established method to study them. We propose a method for the study of the proteins in wastewater overcoming their low concentration and the interference of other molecules. Moreover, we differentiate between the proteins that are soluble and the ones in the particulate. This method is based on concentration, lysis and clean-up steps. The samples were analyzed afterward using liquid chromatography coupled to high-resolution mass spectrometry (HR-LC/MS) and the data searched with Proteome Discoverer. Thus, this complete method has allowed us to characterize the proteomic composition of different wastewater samples with a low volume.
HostingRepository	PRIDE
AnnounceDate	2023-10-24
AnnouncementXML	Submission_2023-10-24_12:58:02.022.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MontserratCarrascal
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-23 12:56:55	ID requested
⏵ 1	2023-10-24 12:58:02	announced
2	2023-11-14 09:10:41	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:10:14	announced	2024-10-22: Updated project metadata.

S, á, nchez-Jim, é, nez E, Abian J, Ginebreda A, Barcel, ó D, Carrascal M, Shotgun proteomics to characterize wastewater proteins. MethodsX, 11():102403(2023) [pubmed]

submitter keyword: proteomics, high-resolution mass spectrometry (HRMS)., Sewage Protein Information Mining (SPIM), wastewater-based epidemiology (WBE),Wastewater, compartmentalized proteome

MontserratCarrascal
contact affiliation	Department of Biological and Environmental Proteomics, Institute of Biomedical Research of Barcelona, Spanish National Research Council (IIBB-CSIC/IDIBAPS), 08036 Barcelona, Spain
contact email	montserrat.carrascal.csic@uab.cat
lab head
MontserratCarrascal
contact affiliation	IIBB-CSIC
contact email	montserrat.carrascal.csic@uab.cat
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042445
     1. Label: PRIDE project
     2. Name: Shotgun Proteomics to characterize wastewater proteins