PXD042402 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Surface Proteomics of ADAM15 Knock-Down cells |
Description | ADAM15 is a member of the disintegrin-metalloproteinase family of sheddases, which plays a role in several biological processes including cartilage homeostasis. Contrary to well-characterized ADAMs, little is known about ADAM15 substrates or how the enzyme exerts its biological functions. Herein, we used “surface-spanning enrichment with click-sugars (SUSPECS)” proteomics to identify ADAM15 substrates and/or proteins regulated by the proteinase at the surface of chondrocyte-like cells. ADAM15-silencing significantly altered membrane levels of 13 proteins, but none of these was a canonical substrate. We used orthogonal techniques to validate ADAM15 effects on 3 of these proteins which have known roles in cartilage homeostasis. This confirmed that ADAM15-silencing increased levels of PDCD1LG2 and reduced levels of vasorin and SLC26A2 through an unknown post-translational mechanism. |
HostingRepository | PRIDE |
AnnounceDate | 2023-06-13 |
AnnouncementXML | Submission_2023-06-13_06:29:38.521.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | StephanMueller |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-05-22 08:45:37 | ID requested | |
⏵ 1 | 2023-06-13 06:29:38 | announced | |
2 | 2023-11-14 08:59:21 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: surface,ADAM15, SUSPECS |
Contact List
Simone DarioScilabra |
contact affiliation | Proteomics Group of Fondazione Ri.MED, Research Department IRCCS ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione), via E. Tricomi 5, 90145 Palermo, Italy |
contact email | sdscilabra@fondazionerimed.com |
lab head | |
StephanMueller |
contact affiliation | DZNE Munich Neuroproteomics |
contact email | stephan.mueller@dzne.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD042402
- Label: PRIDE project
- Name: Surface Proteomics of ADAM15 Knock-Down cells