PXD042381-2

PXD042381 is an original dataset announced via ProteomeXchange.

Title	An integrated multi-omics analysis of identifies distinct molecular characteristics in pulmonary infections of Pseudomonas aeruginosa
Description	Pseudomonas aeruginosa (P. aeruginosa) can cause severe acute infections, including pneumonia and sepsis, and also cause chronic infections commonly in patients with structural respiratory diseases. However, the molecular and pathophysiological mechanisms of P. aeruginosa respiratory infection are largely unknown. Here, we profiled performed to assay for transposase-accessible chromatin using sequencing (ATAC-seq), transcriptomics, and quantitative mass spectrometry-based proteomics and ubiquitin-proteomics in P. aeruginosa-infected lung tissues for multi-omics analysis, while ATAC-seq and transcriptomics were also examined in P. aeruginosa-infected mouse macrophages. To find the pivotal transcription factors that are likely involved in host immune defense, we integrally investigated systematic changes in chromatin accessibility and gene expression in P. aeruginosa-infected lung tissues combined with proteomics and ubiquitin-proteomics studies. We discovered that Stat1 and Stat3 were altered in various omics and found similar results in mouse alveolar macrophages. Taken together, these findings indicate that these crucial transcription factors and their downstream signaling molecules play a critical role in the mobilization of host immune response against P. aeruginosa infection and may serve as potential targets for bacterial infections and inflammatory diseases, as well as provide clear insights and resources for using integrative histological analyses.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:58:18.380.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yang Yang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	ubiquitinylated lysine
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-21 22:54:56	ID requested
1	2023-07-26 20:01:06	announced
⏵ 2	2024-10-22 05:58:18	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Bacterial infection, ATAC-seq, Multi-omics, Pseudomonas aeruginosa, Transcription factor

Yang Yang
contact affiliation	State Key Laboratory of Biotherapy West China Hospital, Sichuan University
contact email	yangyang.roscee@gmail.com
lab head
Yang Yang
contact affiliation	State Key Laboratory of Biotherapy
contact email	yangyang.roscee@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042381
     1. Label: PRIDE project
     2. Name: An integrated multi-omics analysis of identifies distinct molecular characteristics in pulmonary infections of Pseudomonas aeruginosa