PXD042343-2

PXD042343 is an original dataset announced via ProteomeXchange.

Title	Secretome profile of living constructs assembled from cell-to-cell crosslinking of human adipose tissue-derived mesenchymal stromal cells
Description	We report proteomic data from living constructs assembled entirely from human cells covalently tethered via interfacial bridges with a glycosaminoglycan. Here, bioorthogonal azide groups are installed in cell surface glycoproteins via metabolic glycoengineering, allowing for catalyst-free click-chemistry coupling with a complementary hyaluronan biopolymer. This cell-to-cell tethering strategy enables the use of human cells as active crosslinkers and main building blocks of macroscale, tissue-dense living materials. In light of this, we explore the secretome profile of these constructs following a maturation period of 3 days. Bioinformatic analysis and matrisome annotation revealed the presence of a wide range of proteins related to extracellular matrix organization, assembly and remodeling, as well as proteins involved in cell signaling and migration. These findings highlight the potential of this cell-to-cell tethering strategy for creating nascent living tissue mimetics that can be explored in tissue engineering and regenerative medicine endeavors.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:51:18.128.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rui Vitorino
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-18 14:27:47	ID requested
1	2024-07-25 09:45:01	announced
⏵ 2	2024-10-22 06:51:18	announced	2024-10-22: Updated project metadata.

10.1038/S41563-024-01958-1;

submitter keyword: human mesenchymal stromal cells,bioorthogonal click chemistry, metabolic glycoengineering, living materials, secretome.

Joao Mano
contact affiliation	Department of Chemistry, CICECO – Aveiro Institute of Materials, University of Aveiro Lab: COMPASS Research Group
contact email	jmano@ua.pt
lab head
Rui Vitorino
contact affiliation	Universidade de Aveiro
contact email	rvitorino@ua.pt
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD042343

PRIDE project URI

• PRIDE
  1. PXD042343
     1. Label: PRIDE project
     2. Name: Secretome profile of living constructs assembled from cell-to-cell crosslinking of human adipose tissue-derived mesenchymal stromal cells