PXD042332 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Evaluation of the impacts of detergent introduction on the target identification performance of TPP |
Description | In this study, we assessed the target identification performance of TPP in the presence of a commonly used non-ionic detergent or a zwitterionic detergent using a pan-kinase inhibitor staurosporine, our results showed that the addition of either of these detergents significantly impaired the identification performance of TPP at the optimal temperature for soluble target protein identification. Further investigation showed that detergents destabilized the proteome and increased protein precipitation. By lowering the applied temperature point, target identifi-cation performance with detergents is significantly improved and is comparable to that in the absence of detergents. Our findings provide valuable insight into how to select the appropriate temperature range when detergents are used in TPP. In addition, our results also suggest that the combination of detergent and heat may serve as a novel precipitation-inducing force that can be applied for target protein identification. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_09:04:00.066.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | yuying ye |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF; Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-05-18 09:33:19 | ID requested | |
1 | 2023-07-20 12:41:28 | announced | |
⏵ 2 | 2023-11-14 09:04:08 | announced | 2023-11-14: Updated project metadata. |
Publication List
Ye Y, Li K, Ma Y, Zhang X, Li Y, Yu T, Wang Y, Ye M, The Introduction of Detergents in Thermal Proteome Profiling Requires Lowering the Applied Temperatures for Efficient Target Protein Identification. Molecules, 28(12):(2023) [pubmed] |
Keyword List
submitter keyword: mild detergents |
thermal proteome profiling |
target protein identification |
Contact List
Mingling Ye |
contact affiliation | Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS) |
contact email | mingliang@dicp.ac.cn |
lab head | |
yuying ye |
contact affiliation | Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS) |
contact email | yuyingye1809@dicp.ac.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD042332
- Label: PRIDE project
- Name: Evaluation of the impacts of detergent introduction on the target identification performance of TPP