PXD042309-1

PXD042309 is an original dataset announced via ProteomeXchange.

Title	S-Adenosylmethionine Negatively Regulates the Mitochondrial Respiratory Chain Repressor MCJ in the Liver
Description	MCJ (Methylation-Controlled J protein), an endogenous repressor of the mitochondrial respiratory chain, is upregulated in multiple liver diseases but little is known about how it is regulated. S-adenosylmethionine (SAMe), the biological methyl donor, is frequently depleted in chronic liver diseases. Here, we show that SAMe negatively regulates MCJ in the liver. While deficiency in methionine adenosyltransferase alpha 1 (MATα1), enzyme that catalyzes SAMe biosynthesis, leads to hepatic MCJ upregulation, MAT1A overexpression and SAMe treatment reduced MCJ expression. We found that MCJ is methylated at lysine residues and that it interacts with MATα1 in liver mitochondria, likely to facilitate its methylation. Lastly, we observed that MCJ is upregulated in alcohol-associated liver disease, a condition characterized by reduced MAT1A expression and SAMe levels along with mitochondrial injury. MCJ silencing protected against alcohol-induced mitochondrial dysfunction and lipid accumulation. Our study demonstrates a new role of MATα1 and SAMe in reducing hepatic MCJ expression.
HostingRepository	PanoramaPublic
AnnounceDate	2023-05-22
AnnouncementXML	Submission_2023-05-22_20:05:42.913.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lucia Barbier Torres
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	Delta:H(4)C(2)
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-17 21:59:06	ID requested
⏵ 1	2023-05-22 20:05:43	announced

no publication

submitter keyword: MCJ methylation liver

Lucia Barbier Torres
contact affiliation	Cedars-Sinai Medical Center
contact email	lucia.barbiertorres@cshs.org
lab head
Lucia Barbier Torres
contact affiliation	Cedars-Sinai Medical Center
contact email	lucia.barbiertorres@cshs.org
dataset submitter

Panorama Public dataset URI