<<< Full experiment listing


PXD042309 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleS-Adenosylmethionine Negatively Regulates the Mitochondrial Respiratory Chain Repressor MCJ in the Liver
DescriptionMCJ (Methylation-Controlled J protein), an endogenous repressor of the mitochondrial respiratory chain, is upregulated in multiple liver diseases but little is known about how it is regulated. S-adenosylmethionine (SAMe), the biological methyl donor, is frequently depleted in chronic liver diseases. Here, we show that SAMe negatively regulates MCJ in the liver. While deficiency in methionine adenosyltransferase alpha 1 (MATα1), enzyme that catalyzes SAMe biosynthesis, leads to hepatic MCJ upregulation, MAT1A overexpression and SAMe treatment reduced MCJ expression. We found that MCJ is methylated at lysine residues and that it interacts with MATα1 in liver mitochondria, likely to facilitate its methylation. Lastly, we observed that MCJ is upregulated in alcohol-associated liver disease, a condition characterized by reduced MAT1A expression and SAMe levels along with mitochondrial injury. MCJ silencing protected against alcohol-induced mitochondrial dysfunction and lipid accumulation. Our study demonstrates a new role of MATα1 and SAMe in reducing hepatic MCJ expression.
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterLucia Barbier Torres
SpeciesList scientific name: Mus musculus; NCBI TaxID: 10090;
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-05-17 21:59:06ID requested
12023-05-22 20:05:43announced
Publication List
no publication
Keyword List
submitter keyword: MCJ methylation liver
Contact List
Lucia Barbier Torres
contact affiliationCedars-Sinai Medical Center
contact emaillucia.barbiertorres@cshs.org
lab head
Lucia Barbier Torres
contact affiliationCedars-Sinai Medical Center
contact emaillucia.barbiertorres@cshs.org
dataset submitter
Full Dataset Link List
Panorama Public dataset URI