PXD042242 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | RNA Methyltransferase METTL16’s Protein Domains Have Differential Functional Effects on Cell Processes |
| Description | METTL16, a human m6A RNA methyltransferase, contains multiple RNA binding domains and is known to modify U6 and MAT2A RNAs. Usingmutagensis, we generated HEK293 cell lines stabling expressing nutated or WT forms of METTL16 to dtermine the imapct these mutations have on cell processes after removal of endogenous METTL16. We performed bottom-up, untargeted data dependent proteomics analysison all five cell lines to determine the global changes in peptide/protein abdundances; we identified 200-300 statistically significant altered proteinscompared to the wild-type exogenous METTL16 clone. |
| HostingRepository | PRIDE |
| AnnounceDate | 2023-11-14 |
| AnnouncementXML | Submission_2023-11-14_09:11:39.397.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Tonya Zeczycki |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-05-15 15:00:20 | ID requested | |
| 1 | 2023-10-24 13:09:35 | announced | |
| ⏵ 2 | 2023-11-14 09:11:40 | announced | 2023-11-14: Updated project metadata. |
| 3 | 2024-10-22 06:10:27 | announced | 2024-10-22: Updated project metadata. |
Publication List
| Talic ES, Wooten A, Zeczycki TN, Mansfield KD, RNA Methyltransferase METTL16's Protein Domains Have Differential Functional Effects on Cell Processes. Curr Issues Mol Biol, 45(7):5460-5480(2023) [pubmed] |
Keyword List
| submitter keyword: METTLE16 |
Contact List
| Kyle D. Mansfield |
|---|
| contact affiliation | Department of Biochemsitry and Molecular Biology, Brody School of Medicine at East CAraolina University, Greenville, NC USA |
| contact email | mansfieldK@ecu.edu |
| lab head | |
| Tonya Zeczycki |
|---|
| contact affiliation | Brody School of Medicine at ECU |
| contact email | zeczyckit@ecu.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD042242
- Label: PRIDE project
- Name: RNA Methyltransferase METTL16’s Protein Domains Have Differential Functional Effects on Cell Processes
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