PXD042221-2

PXD042221 is an original dataset announced via ProteomeXchange.

Title	Seeding the meiotic DNA break machinery and initiating recombination on chromosome axes
Description	Recombination-mediated linking of homologous chromosomes is a unique meiotic feature that enables the halving of chromosomal ploidy in sexual life cycles. Meiotic recombination is initiated by programmed DNA double-strand breaks (DSBs) that are generated by focal multi-protein clusters that condense onto chromosomal axes by poorly understood mechanisms. We discovered in mice that a DBF4-dependent kinase (DDK)–modulated interaction between IHO1 and the chromosomal axis component HORMAD1 effects the formation of axis-bound IHO1 platforms that enhance nucleation of cytologically distinguishable DSB-machinery clusters. This IHO1-HORMAD1-mediated seeding of the DSB-machinery ensures that sufficient DSBs form for efficient pairing of homologous chromosomes. In the absence of IHO1-HORMAD1 interaction, residual DSB activity depends on ANKRD31, which enhances both the seeding and the growth of DSB-machinery clusters. Thus, meiotic DSBs are ensured by complementing pathways that differentially effect seeding and growth of DSB-machinery clusters, and that synergistically enable assembly of the DSB machinery on chromosomal axes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:31:29.584.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD042221
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Benjamin Neuditschko
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-15 02:18:52	ID requested
1	2024-02-23 02:39:38	announced
⏵ 2	2024-10-22 06:31:30	announced	2024-10-22: Updated project metadata.

10.6019/PXD042221;

submitter keyword: IHO1, Phosphorylation

Franz Herzog
contact affiliation	Institute Krems Bioanalytics, IMC University of Applied Sciences, 3500 Krems, Austria
contact email	franz.herzog@fh-krems.ac.at
lab head
Benjamin Neuditschko
contact affiliation	Institute Krems Bioanalytics, IMC University of Applied Sciences Krems, 3500 Krems, Austria
contact email	benjamin.neuditschko@fh-krems.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD042221

PRIDE project URI

• PRIDE
  1. PXD042221
     1. Label: PRIDE project
     2. Name: Seeding the meiotic DNA break machinery and initiating recombination on chromosome axes