PXD042218 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | PC4-mediated Ku PARylation facilitates NHEJ-dependent DNA damage repair |
Description | Radiotherapy is one of the mainstays for the treatment of hepatocellular carcinoma (HCC); however, a substantial fraction of HCC patients develops radioresistance and eventually suffer from tumour progression or relapse, a major impediment to the use of radiotherapy. One of the main goals in HCC management is to elucidate the mechanisms underlying radioresistance and identify novel therapeutic targets to improve the patients' prognosis. In this study, we report a DNA repair enhancer, human positive cofactor 4 (PC4), that promotes NHEJ-based DNA repair and renders HCC cells resistant to radiation. Mechanistically, PC4 interacts with PARP1 and directs Ku complex PARylation, resulting in successful recruitment of the Ku complex to damaged chromatin and increasing the efficiency of NHEJ repair. Clinically, PC4 is highly expressed in tumour tissues, which is correlated with poor prognosis in HCC patients. Taken together, our data suggest that PC4 is a DNA repair driver that can be targeted to radiosensitize HCC. |
HostingRepository | PRIDE |
AnnounceDate | 2023-10-24 |
AnnouncementXML | Submission_2023-10-24_12:46:15.229.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | QimeiPan |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-05-15 01:37:46 | ID requested | |
⏵ 1 | 2023-10-24 12:46:16 | announced | |
2 | 2023-11-14 09:10:46 | announced | 2023-11-14: Updated project metadata. |
Publication List
Pan Q, Luo P, Shi C, PC4-mediated Ku complex PARylation facilitates NHEJ-dependent DNA damage repair. J Biol Chem, 299(8):105032(2023) [pubmed] |
Keyword List
submitter keyword: PC4, MS, liver cancer |
Contact List
ChummengShi |
contact affiliation | Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University) |
contact email | shicm@tmmu.edu.cn |
lab head | |
QimeiPan |
contact affiliation | Army Medical University |
contact email | mayble_pan@sina.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD042218 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD042218
- Label: PRIDE project
- Name: PC4-mediated Ku PARylation facilitates NHEJ-dependent DNA damage repair