PXD042192-1

PXD042192 is an original dataset announced via ProteomeXchange.

Title	SILAC APMS analysis of SRP9 interactome in HEK-293 cells
Description	The Signal Recognition Particle (SRP) is a universally conserved and abundant ribonucleoprotein particle (RNP) in cells. Its composition and size vary across evolution. In mammals, SRP comprises one RNA molecule, the 7SL RNA, and six proteins: SRP9, SRP14, SRP19, SRP54, SRP68, and SRP72. SRP is essential for targeting transmembrane and secreted proteins to the endoplasmic reticulum. To reveal novel aspects of SRP biogenesis, we established the interactome of the GFP-SRP72 protein. To do so, we have produced stable human U2OS cell line expressing GFP-SRP9 and performed stable isotope labeling by amino acids in cell culture (SILAC) proteomic experiment using GFP-SRP9 as bait.
HostingRepository	PRIDE
AnnounceDate	2024-06-07
AnnouncementXML	Submission_2024-06-07_05:08:48.100.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Franck Vandermoere
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-12 09:43:37	ID requested
⏵ 1	2024-06-07 05:08:48	announced

10.26508/LSA.202402614;

submitter keyword: SRP9, SILAC,APMS, LCMSMS

Severine Massenet
contact affiliation	UMR 7367 CNRS-UL IMoPA Biopôle de l'Université de Lorraine Campus Brabois-Santé 9 avenue de la forêt de Haye 54505 Vandoeuvre-les-Nancy FRANCE
contact email	severine.massenet@univ-lorraine.fr
lab head
Franck Vandermoere
contact affiliation	IGF, CNRS, INSERM, University of Montpellier, Montpellier, France
contact email	franck.vandermoere@igf.cnrs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD042192

PRIDE project URI

• PRIDE
  1. PXD042192
     1. Label: PRIDE project
     2. Name: SILAC APMS analysis of SRP9 interactome in HEK-293 cells