PXD042179-1

PXD042179 is an original dataset announced via ProteomeXchange.

Title	Seeding the meiotic DNA break machinery and initiating recombination on chromosome axes
Description	Recombination-mediated linking of homologous chromosomes is a unique meiotic feature that enables the halving of chromosomal ploidy in sexual life cycles. Meiotic recombination is initiated by programmed DNA double-strand breaks (DSBs) that are generated by focal multi-protein clusters that condense onto chromosomal axes by poorly understood mechanisms. We discovered in mice that a DBF4-dependent kinase (DDK)–modulated interaction between IHO1 and the chromosomal axis component HORMAD1 effects the formation of axis-bound IHO1 platforms that enhance nucleation of cytologically distinguishable DSB-machinery clusters. This IHO1-HORMAD1-mediated seeding of the DSB-machinery ensures that sufficient DSBs form for efficient pairing of homologous chromosomes. In the absence of IHO1-HORMAD1 interaction, residual DSB activity depends on ANKRD31, which enhances both the seeding and the growth of DSB-machinery clusters. Thus, meiotic DSBs are ensured by complementing pathways that differentially effect seeding and growth of DSB-machinery clusters, and that synergistically enable assembly of the DSB machinery on chromosomal axes.
HostingRepository	PRIDE
AnnounceDate	2024-02-23
AnnouncementXML	Submission_2024-02-23_06:42:27.883.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marc Gentzel
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-12 06:07:42	ID requested
⏵ 1	2024-02-23 06:42:28	announced

Dataset with its publication pending

submitter keyword: Biochemical Assay, Meiotic recombination, IHO1-HORMAD1,Protein Phosphorylation, Label-free Quantitative Mass Spectrometry, meiosis

Prof. Attila Toth
contact affiliation	Institute of Physiological Chemistry, Medical Faculty of TU Dresden, Fiedlerstrasse 42 01307 Dresden, Germany
contact email	attila.toth@tu-dresden.de
lab head
Marc Gentzel
contact affiliation	Molecular Analysis - Mass Spectrometry, Center for Molecular and Cellular Bioengineering (CMCB), TU Dresden
contact email	marc.gentzel@tu-dresden.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD042179

PRIDE project URI

• PRIDE
  1. PXD042179
     1. Label: PRIDE project
     2. Name: Seeding the meiotic DNA break machinery and initiating recombination on chromosome axes