PXD042163-1

PXD042163 is an original dataset announced via ProteomeXchange.

Title	Reversible Control of Tetrazine Bioorthogonal Reactivity by Naphthotube mediated Host-Guest Recognition - for Chem
Description	Tetrazine-mediated inverse electron demand Diels-Alder (IEDDA) reactions are widely used ultrafast bioorthogonal reactions, and methods to control such reactions would be extremely useful. Here, we identified a high affinity host-guest pair between synthetic macrocyclic naphthotubes and phenyltetrazine and developed a molecular-recognition strategy for controlling tetrazine reactions by host-caging. Naphthotubes can recognize phenyltetrazine on various biomolecules with low-micromolar and sub-micromolar binding affinities, thus cage their IEDDA reactivity efficiently in a reversible manner. For tetrazine residues on proteins encoded by non-canonical amino acid mutagenesis, their positions could be computationally designed such that exposed residues could be reversibly caged, whereas semiburied residues that were resistant to caging retained their reactivity, thus allowing preparation of site-specific protein dual conjugates and dual protein labeling on living cells with IEDDA reactions. The reactivity of tetrazine can be further regulated in living animals. Our strategy is thus expected to expand the applications of tetrazine chemistry.
HostingRepository	iProX
AnnounceDate	2023-05-11
AnnouncementXML	Submission_2023-05-11_21:38:30.766.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haoyu Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; oxidation
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-11 21:38:21	ID requested
⏵ 1	2023-05-11 21:38:31	announced

Dataset with its publication pending

submitter keyword: host-guest recognition

bioorthogonal reaction

genetic code expansion

tetrazine

bioconjugation

cell imaging

Tao Liu
contact affiliation	Peking University
contact email	taoliupku@pku.edu.cn
lab head
Haoyu Wang
contact affiliation	Peking University
contact email	haoyuwangpku@pku.edu.cn
dataset submitter

iProX dataset URI