PXD042156-2

PXD042156 is an original dataset announced via ProteomeXchange.

Title	Impact of STAU1 depletion on the protein expression profile of rhabdomyosarcoma cell lines
Description	Two human rhabdomyosarcoma cell lines (RD, RH36, RH30, and RH18) and one none-transformed skeletal muscle cell line (HSMM-C3) cell line were studied for the impact of Staufen1 depletion on their protein expression profile. Proteomics was performed for control and Staufen1 knockdown cells (n=3). For this, we generated STAU1 knockdown cell lines using a mixture of three independent STAU1-targeting lentiviral-shRNA constructs. cells were cultured for 72 hours and then protein extraction was performed as follow: Cells were lysed in 1X RIPA buffer (20 mM Tris-HCl (pH 7.5), 150mM NaCl, 1mM Na2- EDTA, 1mM EGTA, 1% NP-40, 1% sodium deoxycholate, 2.5 mM sodium pyrophosphate, 1mM β-glycerophosphate, 1mM Na3 VO4, 1µg/ml leupeptin). Protein concentration was tested according to the BCA assay protocol (Thermofisher Scientific) and samples were used in proteomics experiment.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:52:30.123.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shekoufeh Almasi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-11 18:10:50	ID requested
1	2023-10-22 12:06:08	announced
⏵ 2	2023-11-14 07:52:30	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:06:47	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

ProteomeXchange project tag: Cancer (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: Embryonal Rhabdomyosarcoma, Staufen1

Bernard J. Jasmin
contact affiliation	Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, K1H 8M5, Canada
contact email	jasmin@uottawa.ca
lab head
Shekoufeh Almasi
contact affiliation	University of Ottawa
contact email	sh677216@dal.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042156
     1. Label: PRIDE project
     2. Name: Impact of STAU1 depletion on the protein expression profile of rhabdomyosarcoma cell lines