PXD042150-1

PXD042150 is an original dataset announced via ProteomeXchange.

Title	Overcoming intra-tumoral heterogeneity for biomarker discovery in the High-Grade Serous Ovarian Cancer proteome
Description	High-grade serous ovarian cancer (HGSC) has a disproportionate impact on cancer-related mortality in women. There is an urgent need to improve treatment options for HGSC. This includes better selection of patients who will benefit from existing treatments, including HRD targeted therapy, and discovery of new targeted therapeutic options. Reliable tissue-based biomarkers that can guide treatment selection are key to progress in this area. A challenge for their definition is the large volume of disease that is characteristically present at HGSC diagnosis and that can show considerable heterogeneity within and between anatomical sites. In order to address this issue, we carried out a detailed study of the HGSC proteome in eleven individuals by comparing genomic and gene-expression features with multiple independent proteomic results derived from fresh frozen (FF) and formalin fixed paraffin embedded (FFPE) samples from both ovary and a common metastatic site, omentum. Our findings gave emphasis to the influence of sample site of origin on biomarker expression, and revealed opportunities to detect mechanisms shaping the HGSC tumour immune microenvironment from tissue proteomics. The dataset also forms a useful resource to investigate molecular heterogeneity in HGSC.
HostingRepository	PRIDE
AnnounceDate	2025-06-13
AnnouncementXML	Submission_2025-06-13_06:38:33.155.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Srinivas Srikanth Manda
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-11 08:13:06	ID requested
⏵ 1	2025-06-13 06:38:34	announced

Manda SS, Espersen MM, Mapagu C, Bouantoun N, Boros J, Chiew YE, Srirangan S, Pattnaik S, Kennedy CJ, Brand AH, Garsed DW, Pandey A, Bowtell DDL, Lucas N, Xavier D, Mahboob S, Bucio-Noble D, Tully B, Hains PG, Robinson PJ, Zhong Q, Reddel R, DeFazio A, Balleine RL, Overcoming intra-tumoral heterogeneity for biomarker discovery in the high-grade serous ovarian cancer proteome. NPJ Precis Oncol, 9(1):172(2025) [pubmed]

10.1038/s41698-025-00911-y;

submitter keyword: HGSOC, cGAS-STING, heterogeneity, muti-omics, SWATH-MS

Rosemary Balleine
contact affiliation	University of Sydney
contact email	rosemary.balleine@sydney.edu.au
lab head
Srinivas Srikanth Manda
contact affiliation	Children's Medical Research Institute, CMRI, Westmead
contact email	smanda@cmri.org.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD042150

PRIDE project URI

• PRIDE
  1. PXD042150
     1. Label: PRIDE project
     2. Name: Overcoming intra-tumoral heterogeneity for biomarker discovery in the High-Grade Serous Ovarian Cancer proteome