PXD042130-1

PXD042130 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Disruption of the Productive Encounter Complex Results in Dysregulation of DIAPH1 Activity
Description	Formins are characterized by a N-terminal regulatory domain, which binds to the C-terminal auto-inhibitory domain, yielding an inactive complex. Cross-Linking and tandem MS was used to characterized the disordered "RRKR" motif of the C-terminus, which is notably absent from solved crystallographic structures. These results were used with NMR spectroscopy to locate and understand the dynamics of this disordered region. The proteins were synthesized using recombinant techniques and purified using chromatographic techniques. Proteins were mixed in equi-molar concentrations with excess concentration of cross-linkers. Samples were analyzed using SDS-PAGE gels, excised for chemical processing, and subjected to Trypsin digestion. Resulting peptides were extracted using formic acid and acetonitrile, lyophilized, and stored until MS analysis
HostingRepository	jPOST
AnnounceDate	2023-05-11
AnnouncementXML	Submission_2023-05-10_13:22:54.341.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gregory Theophall
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-10 13:22:50	ID requested
⏵ 1	2023-05-10 13:22:54	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: actin polymerization, diabetes, intrinsically disordered regions, protein-protein interactions, NMR, mass spectrometry, cross-linking, encounter complex, formins, receptor for advanced glycation end products, mDia1, DIAPH1.

submitter keyword: actin polymerization, diabetes, intrinsically disordered regions, protein-protein interactions, NMR, mass spectrometry, cross-linking, encounter complex, formins, receptor for advanced glycation end products, mDia1, DIAPH1.

Contact List

Alexander Shekhtman
lab head
Gregory Theophall
contact affiliation	SUNY Albany
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002156/