PXD042126-1

PXD042126 is an original dataset announced via ProteomeXchange.

Title	Lipid-Associated Macrophages Reshape BAT Cell Identity in Obesity
Description	Obesity and type 2 diabetes cause a loss in brown adipose tissue (BAT) activity in mice and human, but the molecular mechanisms that drive BAT cell remodeling remain largely. Using a multilayered approach, we comprehensively map a deep reorganization in the BAT cells. We uncovered a subset of macrophages as the lipid-associated macrophages (LAM), which were massively increased in genetic and dietary model of BAT expansion. LAM participate in this scenario by capturing extracellular vesicles carrying damaged lipids and mitochondria released from metabolically-stressed brown adipocytes. CD36 scavenger receptor drives LAM phenotype and through in vitro and in vivo models, we demonstrated that CD36-deficient LAM increased brown fat genes. LAM release Tgf1 that reduces brown adipocytes identity through Aldh1a1 induction. This study provides the first description of cell dynamics in BAT of obese models identifying LAM as responder to tissue-level metabolic stress and key driver to loss of BAT cell identity.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_09:36:40.257.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniele Lettieri Barbato
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-10 10:29:52	ID requested
⏵ 1	2025-05-06 09:36:41	announced

Sciarretta F, Ninni A, Zaccaria F, Chiurchi, ù V, Bertola A, Karlinsey K, Jia W, Ceci V, Di Biagio C, Xu Z, Gaudioso F, Tortolici F, Tiberi M, Zhang J, Carotti S, Boudina S, Grumati P, Zhou B, Brestoff JR, Ivanov S, Aquilano K, Lettieri-Barbato D, Lipid-associated macrophages reshape BAT cell identity in obesity. Cell Rep, 43(7):114447(2024) [pubmed]

10.1016/j.celrep.2024.114447;

submitter keyword: Macrophages,BAT, Lipid, Obesity

Daniele Lettieri Barbato
contact affiliation	Department of Biology, University of Rome Tor Vergata, Rome, Italy
contact email	daniele.lettieri.barbato@uniroma2.it
lab head
Daniele Lettieri Barbato
contact affiliation	University of Rome "Tor Vergata"
contact email	aquilettlab@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD042126

PRIDE project URI

• PRIDE
  1. PXD042126
     1. Label: PRIDE project
     2. Name: Lipid-Associated Macrophages Reshape BAT Cell Identity in Obesity