PXD042114-1

PXD042114 is an original dataset announced via ProteomeXchange.

Title	Unravelling the function of GEXP15, a regulator of Protein Phosphatase type 1, in Plasmodium falciparum
Description	The Protein Phosphatase type 1 catalytic subunit (PP1c) works in combination with an array of regulatory proteins to specifically dephosphorylate a diverse range of substrates in the cell. In the human malaria parasite, Plasmodium falciparum, this phosphatase and its regulators are poorly characterized. In this study, we employed a multi-faceted approach to investigate the structural and functional characteristics of a conserved Plasmodium specific regulator known as Gametocyte EXported Protein 15, GEXP15. In-silico study highlights three significant regions of interest, an PP1-interacting RVxF motif located in its N-terminal region, a conserved domain whose function is unknown, and a GYF-like domain that may mediate specific protein-protein interactions. In vitro interaction studies shows that GEXP15 has a direct interaction with PP1 via the RVxF binding motif, enhancing its phosphatase activity. Using transgenic GEXP15-tagged line, confocal microscopy reveals that GEXP15 is highly expressed in late asexual stages and is localized in the parasite nucleus. Immunoprecipitation assays followed by mass spectrometry analyses shows its interaction with ribosomal and RNA binding proteins. Furthermore, pulldown analyses of His-tagged recombinant functional domains of GEXP15 confirm its binding to the ribosomal complex via the GYF domain. Our study provides the first insight into the PfGEXP15-PP1-ribosome interaction, crucial for protein translation, and supports PfGEXP15 as a potential target for malaria drug development.
HostingRepository	PRIDE
AnnounceDate	2023-10-24
AnnouncementXML	Submission_2023-10-24_13:04:21.626.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chiaraguerrera
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Plasmodium falciparum (isolate 3D7); NCBI TaxID: 36329;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-10 05:04:30	ID requested
⏵ 1	2023-10-24 13:04:22	announced
2	2023-11-14 09:10:46	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:10:14	announced	2024-10-22: Updated project metadata.

Mansour H, Cabezas-Cruz A, Peucelle V, Farce A, Salom, é, -Desnoulez S, Metatla I, Guerrera IC, Hollin T, Khalife J, . Int J Mol Sci, 24(16):(2023) [pubmed]

submitter keyword: CD2BP2, Malaria, GYF domain,Protein Phosphatase 1, Ribosome biogenesis, Plasmodium, GEXP15

Ida ChiaraGuerrera
contact affiliation	Proteomics platform Necker, Université Paris Cité - Structure Fédérative de Recherche Necker, INSERM US24/CNRSUAR3633, Paris 75015, France.
contact email	chiara.guerrera@inserm.fr
lab head
Chiaraguerrera
contact affiliation	Necker proteomics, INSERM
contact email	chiara.guerrera@inserm.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042114
     1. Label: PRIDE project
     2. Name: Unravelling the function of GEXP15, a regulator of Protein Phosphatase type 1, in Plasmodium falciparum