PXD042085-1

PXD042085 is an original dataset announced via ProteomeXchange.

Title	IP-mass spectrometry of MeCP2 binding proteins during cell cycle re-entry
Description	To reveal the proteins of the ubiquitination system possibly interacting with MeCP2, NIH3T3 cells were synchronized by serum starvation for 36 hours and collected following 15% FBS stimulation for 3 or 9 hours. Total protein (1 mg) was incubated with anti-Mecp2 (5 μg) overnight at 4 °C with constant mixing. IgG was used as the negative control. Then, antibody was incubated with Dynabeads for 2 h with shaking. After three washings, retained proteins were eluted using 30 μl of SDS lysis buffer. Eluted Mecp2-associated cellular proteins were separated by SDS-PAGE and stained with Coomassie blue. Trypsin was used to digest stained protein bands. An Orbitrap Elite mass spectromete was used to analyze the digested samples by Applied Protein Technology Co., Ltd. (Shanghai, China). Using Mascot as a search engine, fragment spectra were scanned against the Uniprot database to identify proteins.
HostingRepository	PRIDE
AnnounceDate	2024-04-26
AnnouncementXML	Submission_2024-04-26_00:43:44.376.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zeyou Qiu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-09 06:02:22	ID requested
⏵ 1	2024-04-26 00:43:44	announced

10.7554/ELIFE.89912.1;

submitter keyword: MeCP2, IP-Mass, G0

Jun Yang
contact affiliation	Department of Cell Biology，Southern Medical University
contact email	junyang@smu.edu.cn
lab head
Zeyou Qiu
contact affiliation	Southern Medical University
contact email	qzy199607@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042085
     1. Label: PRIDE project
     2. Name: IP-mass spectrometry of MeCP2 binding proteins during cell cycle re-entry