PXD042084-1

PXD042084 is an original dataset announced via ProteomeXchange.

Title	Differential phosphoproteomics upon chitin-treatment in Marchantia polymorpha
Description	Pattern-recognition receptor (PRR)-triggered immunity (PTI) plays a pivotal role in plant immunity to ward off a wide range of pathogenic microbes. The model liverwort Marchantia polymorpha is gaining popularity in investigating the evolution of plant-microbe interactions. The M. polymorpha is capable of triggering defense-related gene expression by sensing components in bacterial and fungal extracts, suggesting existence of PTI in this plant model. However, the molecular components that would form PTI in M. polymorpha have not yet been described. We show that, in M. polymorpha, lysin motif (LysM) receptor-like kinase (LYK) MpLYK1 and LYK-related (LYR) MpLYR, among four LysM receptor homologs, are required for sensing chitin and peptidoglycan (PGN) fragments and thereby triggering a series of immune responses. Phosphoproteomic analysis of M. polymorpha in response to chitin treatment comprehensively identified regulatory proteins that would shape LysM-mediated PTI. The identified proteins covered homologs of well-described PTI components in angiosperms as well as proteins whose roles in PTI are not yet determined including the blue-light receptor phototropin MpPHOT. We revealed that MpPHOT is required for a negative feedback of defense-related gene expression during PTI. Taken together, this study provides the basic framework of LysM-mediated PTI in M. polymorpha and demonstrates the utility of M. polymorpha as a plant model for discovering novel or fundamental molecular mechanisms underlying PRR-triggered immune signaling in plants.
HostingRepository	PRIDE
AnnounceDate	2023-11-06
AnnouncementXML	Submission_2023-11-06_02:10:01.915.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sara ChristinaStolze
SpeciesList	scientific name: Marchantia polymorpha; NCBI TaxID: 3197;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-09 05:27:08	ID requested
⏵ 1	2023-11-06 02:10:02	announced
2	2023-11-14 08:19:33	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:13:40	announced	2024-10-22: Updated project metadata.

submitter keyword: pattern-triggered immunity, phosphoproteomics, Marchantia polymorpha

HirofumiNakagami
contact affiliation	Basic Immune System of Plants / Protein Mass Spectrometry Max Planck Institute for Plant Breeding Research Carl-von-Linne-Weg 10, 50829 Cologne, Germany
contact email	nakagami@mpipz.mpg.de
lab head
Sara ChristinaStolze
contact affiliation	Plant Proteomics and Mass Spectrometry Group Max Planck Institute for Plant Breeding Research Carl-von-Linné Weg 10 50829 Cologne
contact email	stolze@mpipz.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042084
     1. Label: PRIDE project
     2. Name: Differential phosphoproteomics upon chitin-treatment in Marchantia polymorpha