PXD042083-2

PXD042083 is an original dataset announced via ProteomeXchange.

Title	Pre-treatment of Interferon-α stimulates DHX58 to prevent hepatic ferroptosis
Description	Characterized by lethal iron accumulation and lipid peroxidation, ferroptosis plays critical roles in liver injury, especially caused by ischemia/reperfusion (I/R) of hepatic inflow occlusion during liver operation. However, the lack of effective and safe clinical precautionary measure is still the main problem in preventing hepatic ferroptosis. Here, we found that the excessive production of reactive oxygen species could decrease the expression of Interferon (IFN)-stimulated gene DExH-box helicase 58 (DHX58) in hepatocytes, and then promote hepatic ferroptosis, while pre-treatment using IFN-α increased DHX58 expression and prevented ferroptosis during I/R injury. Mechanistically, DHX58 with RNA-binding activity could constitutively associate the mRNA of glutathione peroxidase 4 (GPX4), a crucial ferroptosis suppressor, and then recruit the m6A reader YT521-B homology domain containing 2 (YTHDC2) to promote the translation of Gpx4 mRNA in m6A-dependent manner, thus enhancing GPX4 protein level and preventing hepatic ferroptosis. Therefore, we provide mechanistic evidence for the IFN-stimulated DHX58 in promoting the translation of m6A-modified Gpx4 mRNA, and suggest the promising clinical potential of IFN-α pre-treatment in the prevention of hepatic ferroptosis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:42:29.341.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jia Kaiwei
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-09 04:02:54	ID requested
1	2024-05-24 02:13:12	announced
⏵ 2	2024-10-22 06:42:30	announced	2024-10-22: Updated project metadata.

Jia KW, Yao RQ, Fan YW, Zhang DJ, Zhou Y, Wang MJ, Zhang LY, Dong Y, Li ZX, Wang SY, Wang M, Li YH, Zhang LX, Lei T, Gui LC, Lu S, Yang YY, Wang SX, Yu YZ, Yao YM, Hou J, stimulates DExH-box helicase 58 to prevent hepatocyte ferroptosis. Mil Med Res, 11(1):22(2024) [pubmed]

10.1186/s40779-024-00524-9;

submitter keyword: Interferon

DHX58

GPX4

m6A modification

YTHDC2

Ferroptosis

Kaiwei jia
contact affiliation	National Key Laboratory of Medical Immunology & Institute of Immunology, Second Military Medical University
contact email	kwjia1994@126.com
lab head
Jia Kaiwei
contact affiliation	National Key Laboratory of Medical Immunology, Institute of Immunology, Second Military Medical University, Shanghai, China
contact email	531593244@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD042083

PRIDE project URI

• PRIDE
  1. PXD042083
     1. Label: PRIDE project
     2. Name: Pre-treatment of Interferon-α stimulates DHX58 to prevent hepatic ferroptosis