PXD042039-1
PXD042039 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | New avenues for human blood plasma biomarker discovery via improved in-depth analysis of the low-abundant N-glycoproteome |
Description | An in-depth N-glycoproteomic analysis of human blood plasma was achieved by the combination of a top 14-High Abundant Protein (HAP) depletion step and protein fractionation via a GLEFrEE system. The sample preparation workflow included a tryptic digestion and glycopeptide enrichment, followed by an intact N-glycopeptide LC-MS/MS-based analysis, established by us. Two MS/MS acquisitions were applied using different fragmentation energies HCD.step and HCD.low (HCD: Higher Energy Collision Dissociation). A proteomic analysis was conducted, to assess the gain in the identification of middle- and low-abundant proteins. The protein search was applied on the three stages of the workflow: 1) untreated blood plasma, 2) top 14-HAP depleted sample, and 3) top 14-HAP depleted and GELFrEE fractionated sample. (Search engine Proteome Discoverer v 2.5) An N-glycoproteomic analysis was performed on the HCD.low and HCD.step raw data from the top 14-HAP depleted and GELFrEE fractionated sample (search engine Byonic v4.2.10). No FDR cuts nor decoys search was applied and manual validation was conducted instead. After the validation, we achieved the identification of 1929 N-glycopeptides, 942 N-glycosylation sites, and 805 glycoproteins. |
HostingRepository | MassIVE |
AnnounceDate | 2023-05-27 |
AnnouncementXML | Submission_2023-05-27_01:47:06.684.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Frania Jaqueline Zuniga Banuelos |
SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
ModificationList | unknown modification |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-05-06 10:03:28 | ID requested | |
⏵ 1 | 2023-05-27 01:47:07 | announced |
Publication List
no publication |
Keyword List
submitter keyword: data validation, disialyl Lewis C epitope, intact N-glycopeptides, glucuronidated glycan, glycoproteomic workflow, human blood plasma, low-abundant protein, phosphorylated glycan, protein fractionation, rare glycan, sulfated glycan |
Contact List
Frania Jaqueline Zuniga-Banuelos | |
---|---|
contact affiliation | Max Planck Institute for Dynamics of Complex Technical Systems |
contact email | zuniga@mpi.magdeburg.de |
lab head | |
Erdmann Rapp | |
contact affiliation | Max Planck Institute for Dynamics of Complex Technical Systems |
contact email | rapp@mpi-magdeburg.mpg.de |
lab head | |
Udo Reichl | |
contact affiliation | Max Planck Institute for Dynamics of Complex Technical Systems |
contact email | ureichl@mpi-magdeburg.mpg.de |
lab head | |
Marcus Hoffmann | |
contact affiliation | Max Planck Institute for Dynamics of Complex Technical Systems |
contact email | mhoffmann@mpi-magdeburg.mpg.de |
lab head | |
Frania Jaqueline Zuniga Banuelos | |
contact affiliation | Max Planck Institute |
contact email | zuniga@mpi-magdbeurg.mpg.de |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000091870/ |