PXD042038-1

PXD042038 is an original dataset announced via ProteomeXchange.

Title	4D-FastDIA quantitative phosphorylation modification omics analysis before and after expression of Salmonella effector SteC in RAW264.7 cell
Description	The Salmonella effector SteC is the only protein kinase encoded by Salmonella pathogenicity island 2 that is secreted through the type III secretion system. SteC is known to trigger actin rearrangement via the phosphorylated MEK pathway, and our previous experiments demonstrated that the migration process of macrophages found during Salmonella infection is dependent on the rearrangement of the host cell actin backbone and the action of SteC.To further investigate the target of SteC in the host, we constructed a SteC-RAW264.7 cell line and performed phosphomics analysis using 4D-FastDIA to identify the direct substrates of SteC that trigger macrophage migration and lead to cytoskeletal rearrangement.
HostingRepository	PRIDE
AnnounceDate	2024-02-22
AnnouncementXML	Submission_2024-02-21_18:02:40.044.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Min Zhang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	timsTOF

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-06 02:45:16	ID requested
⏵ 1	2024-02-21 18:02:40	announced

Dataset with its publication pending

submitter keyword: Salmonella,4D-FastDIA,SteC,phosphoomics

Min Zhang
contact affiliation	Department of Pathogen Biology, School of Clinical and Basic Medicine, Shandong First Medical University &Shandong Academy of Medical Sciences
contact email	15662004814@163.com
lab head
Min Zhang
contact affiliation	Shandong First Medical University & Shandong Academy of Medical Sciences
contact email	15662004814@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD042038

PRIDE project URI

• PRIDE
  1. PXD042038
     1. Label: PRIDE project
     2. Name: 4D-FastDIA quantitative phosphorylation modification omics analysis before and after expression of Salmonella effector SteC in RAW264.7 cell