PXD042024-1

PXD042024 is an original dataset announced via ProteomeXchange.

Title	Vitamin D binding protein induces skeletal muscle atrophy and contributes to cancer-associated muscle wasting
Description	The maintenance of skeletal muscle tissue is of pivotal importance in health and disease, as its loss is often associated with chronic or progressive pathologies, generally worsening the prognosis. Increased levels of vitamin D binding protein (VDBP) in biological samples were reported to co-occur with several pathologies susceptible to muscle wasting, including various types of tumors. We, therefore, hypothesized that VDBP may participate in muscle wasting and investigated its direct effects on skeletal muscle homeostasis. Here we demonstrate that VDBP induces atrophy independently of vitamin D. In C2C12 myotubes, we identified the perturbation of the intracellular actin dynamic and subsequent mitochondrial dysfunction as the main molecular mechanisms at the basis of VDBP-induced atrophy. Coherently, the ectopic introduction of VDBP in mice lacking the protein (Gc-knockout mice; Gc-KO) induces muscle atrophy, the dismantling of neuromuscular junctions, and decreased force. Finally, we provide proof-of-concept evidence that VDBP contributes to cancer-associated muscle wasting. Altogether, these findings provide novel insights into the biological function of VDBP and have potential implications for the treatment of muscle wasting.
HostingRepository	PRIDE
AnnounceDate	2026-02-20
AnnouncementXML	Submission_2026-02-20_05:00:35.084.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marcello Manfredi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-05 09:45:06	ID requested
⏵ 1	2026-02-20 05:00:35	announced

Dataset with its publication pending

submitter keyword: cancer, proteomics,VDBP, skeletal muscle atrophy

Marcello Manfredi
contact affiliation	Biological Mass Spectrometry Lab Department of Translational Medicine (DiMeT) Center for Translational Research on Autoimmune & Allergic Diseases - CAAD University of Piemonte Orientale Novara, Italy
contact email	marcello.manfredi@uniupo.it
lab head
Marcello Manfredi
contact affiliation	University of Eastern Piedmont
contact email	marcello.manfredi@uniupo.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042024
     1. Label: PRIDE project
     2. Name: Vitamin D binding protein induces skeletal muscle atrophy and contributes to cancer-associated muscle wasting