PXD042014 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | TurboID-based proxisome mapping of the human glucocorticoid receptor in A549 cells |
Description | Exogenous glucocorticoids are frequently used to treat inflammatory disorders and as adjuncts in solid cancers. However, their use is associated with severe side effects and therapy resistance. Novel glucocorticoid receptor (GR) ligands with a patient-validated reduced side effect profile have not yet reached the clinic. GR is a member of the nuclear receptor family of transcription factors and heavily relies on interactions with coregulator proteins for its transcriptional activity. To elucidate the role of the GR interactome in the altered transcriptional activity of GR following treatment with agonists, antagonists or lead selective GR agonists and modulators (SEGRAMs), we generated comprehensive interactome maps by high-confidence proximity proteomics in lung epithelial carcinoma cells. We found that the GR antagonist RU486 and the SEGRAM Dagrocorat both reduced GR interaction with CREB-binding protein (CBP)/p300 and the mediator complex compared to the full agonist Dexamethasone. Our data offer new insights into the role differential coregulator protein recruitment in shaping specific GR-mediated transcriptional responses. |
HostingRepository | PRIDE |
AnnounceDate | 2024-05-24 |
AnnouncementXML | Submission_2024-05-24_03:00:47.261.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD042014 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | An Staes |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-05-05 04:19:09 | ID requested | |
⏵ 1 | 2024-05-24 03:00:48 | announced | |
Publication List
Van Moortel L, Verhee A, Thommis J, Houtman R, Melchers D, Delhaye L, Van Leene C, Hellemans M, Gevaert K, Eyckerman S, De Bosscher K, Selective Modulation of the Human Glucocorticoid Receptor Compromises GR Chromatin Occupancy and Recruitment of p300/CBP and the Mediator Complex. Mol Cell Proteomics, 23(3):100741(2024) [pubmed] |
10.6019/PXD042014; |
10.1016/j.mcpro.2024.100741; |
Keyword List
submitter keyword: LC-MSMS, TurboID,Glucocorticoid receptor, interactomics |
Contact List
Karolien De Bosscher |
contact affiliation | 1 VIB-UGent Center for Medical Biotechnology, B9052 Ghent, Belgium; 2 Department of Biomolecular Medicine, Ghent University, B9052 Ghent, Belgium. |
contact email | karolien.debosscher@vib-ugent.be |
lab head | |
An Staes |
contact affiliation | Medical Protein Chemistry |
contact email | an.staes@vib-ugent.be |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD042014
- Label: PRIDE project
- Name: TurboID-based proxisome mapping of the human glucocorticoid receptor in A549 cells