PXD041978-1

PXD041978 is an original dataset announced via ProteomeXchange.

Title	Profiling of the Helicobacter pylori redox switch HP1021 regulon using a multi-omics approach
Description	The gastric human pathogen Helicobacter pylori has developed mechanisms to combat stress factors, including reactive oxygen species (ROS). Here, we present a comprehensive study on the redox switch protein HP1021 regulon combining transcriptomic, proteomic and DNA-protein interactions analyses. Our results indicated that HP1021 modulates H. pylori's response to oxidative stress. HP1021 controlled the transcription of 497 genes, including 407 genes related to response to oxidative stress. 79 proteins were differently expressed in the HP1021 deletion mutant. HP1021 regulated typical ROS response pathways (katA, rocF) and less canonical ones, particularly DNA uptake and central carbohydrate metabolism. We identified HP1021 as the first molecular regulator of competence in H. pylori, as HP1021-dependent repression of the comB DNA uptake genes was relieved under oxidative conditions, increasing natural competence. Furthermore, HP1021 controlled glucose consumption by directly regulating the gluP transporter and had an important impact on maintaining the energetic balance in the cell.
HostingRepository	PRIDE
AnnounceDate	2023-09-20
AnnouncementXML	Submission_2023-09-20_06:34:18.854.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ChristinaLudwig
SpeciesList	scientific name: Helicobacter pylori J99 (Campylobacter pylori J99); NCBI TaxID: 85963;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-04 05:59:55	ID requested
⏵ 1	2023-09-20 06:34:19	announced
2	2024-10-22 06:03:28	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: glucose consumption,Helicobacter pylori, HP1021, oxidative stress

ChristinaLudwig
contact affiliation	Bavarian Center for Biomolecular Mass Spectrometry (BayBioMS), Technical University of Munich (TUM), 85354 Freising, Germany
contact email	tina.ludwig@tum.de
lab head
ChristinaLudwig
contact affiliation	TU Munich
contact email	tina.ludwig@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD041978

PRIDE project URI

• PRIDE
  1. PXD041978
     1. Label: PRIDE project
     2. Name: Profiling of the Helicobacter pylori redox switch HP1021 regulon using a multi-omics approach