PXD041951-1

PXD041951 is an original dataset announced via ProteomeXchange.

Title	Flavodoxin Landscape in Clostridioides difficile
Description	C. difficile infections have become a major challenge in medical facilities today. The bacterium is capable of spore formation, which even allows survival of antibiotic treatment. Therefore, research on the physiology of C. difficile is important for the development of alternative treatment strategies. In this study, we investigated eight putative flavodoxins of C. difficile 630. Flavodoxins are small electron transfer proteins of specifically low potential. The unusually high number of flavodoxins in C. difficile compared to other bacteria suggests that they are expressed under different conditions. Therefore, we investigated expression along bacterial growth and found high transcription levels during the exponential growth phase, especially for floX. Since flavodoxins in other bacteria are capable of replacing ferredoxins with their [2Fe-2S] clusters under iron deficiency conditions, we also examined flavodoxin expression in C. difficile under low iron and no iron levels.
HostingRepository	PRIDE
AnnounceDate	2024-02-02
AnnouncementXML	Submission_2024-02-02_00:53:56.669.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniela Zuehlke
SpeciesList	scientific name: Peptoclostridium difficile (strain 630) (Clostridium difficile); NCBI TaxID: 272563;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-03 09:15:44	ID requested
⏵ 1	2024-02-02 00:53:57	announced
2	2024-10-22 06:28:23	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: expression profiling,mass spectrometry, parallel reaction monitoring

Susanne Sievers
contact affiliation	University of Greifswald, Institute of Microbiology
contact email	susanne.sievers@uni-greifswald.de
lab head
Daniela Zuehlke
contact affiliation	University of Greifswald, Institute of Microbiology
contact email	zuehlke@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041951
     1. Label: PRIDE project
     2. Name: Flavodoxin Landscape in Clostridioides difficile