PXD041931 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Exploring Diverse Clinical Presentations in COVID-19 through Circulating Extracellular Vesicle Proteomics Analysis |
Description | Extracellular vesicles (EVs) released by virus-infected cells are susceptible to incorporate viral peptides. Here, we hypothesized that the molecular characterization of EVs obtained from COVID-19 patients will reveal novel immunogenic viral peptides. Blood from COVID-19 patients with severe (G1), mild/asymptomatic disease (G2) and controls (G3), were collected during active infection (m0) in early 2020 and eight months (m8) post-infection. Clinical data showed increased inflammation markers in G1. Neutralizing antibodies in non-vaccinated G1 individuals increased at m8, indicating sustained exposure to viral antigens. Proteomic profiling of EVs isolated by three different methods, immunocapture (CD9), ganglioside-capture (Siglec-1) and size-exclusion chromatography (SEC), failed in identifying viral peptides. These results were validated by Western blot against Spike-RBD and EV proteomics of hamsters with induced viral infection. Noticeably, G3 individuals showed dramatic changes in EV-associated protein abundance when comparing m0-m8, likely due to vaccination. Human EV cargo identified proteins with prognostic potential in severe disease. |
HostingRepository | PRIDE |
AnnounceDate | 2024-09-25 |
AnnouncementXML | Submission_2024-09-25_05:05:11.296.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Joan Josep Bech-Serra |
SpeciesList | scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos; Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-05-02 10:33:17 | ID requested | |
⏵ 1 | 2024-09-25 05:05:11 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: proteomics profiling,COVID-19 patients, clinical data, ganglioside-capture(siglec-1), antibody response, extracellular vesicles, size-exclusion chromatography(SEC), Tandem mass tag(TMT), immunocapture(CD9) |
Contact List
Hernando A. |
contact affiliation | ISGlobal, Hospital ClĂnic - Universitat de Barcelona, Barcelona, Spain |
contact email | hernandoa.delportillo@isglobal.org |
lab head | |
Joan Josep Bech-Serra |
contact affiliation | Proteomics Unit - Josep Carreras Leukaemia Research Institute |
contact email | jbech@carrerasresearch.org |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041931
- Label: PRIDE project
- Name: Exploring Diverse Clinical Presentations in COVID-19 through Circulating Extracellular Vesicle Proteomics Analysis