PXD041898-1

PXD041898 is an original dataset announced via ProteomeXchange.

Title	XCP1 cleaves Pathogenesis-related protein 1 into CAPE9 for systemic immunity in Arabidopsis
Description	This repository is related to the work” XCP1 cleaves Pathogenesis-related protein 1 into CAPE9 for systemic immunity in Arabidopsis”. In this study, we found that the C-terminal proteolytic processing of a caspase-like substrate motif “CNYD” within Pathogenesis-related protein 1 (PR1) generates an immunomodulatory cytokine (CAPE9) in Arabidopsis. Salicylic acid enhances CNYD-targeted protease activity and the proteolytic release of CAPE9 from PR1 in Arabidopsis. This process involves a protease exhibiting caspase-like enzyme activity, identified as Xylem cysteine peptidase 1 (XCP1). XCP1 exhibits a calcium-modulated pH-activity profile and a comparable activity to human caspases. XCP1 is required to induce systemic immunity triggered by pathogen-associated molecular patterns. This work reveals XCP1 as a key protease for plant immunity, which produces the cytokine CAPE9 from the canonical salicylic acid signaling marker PR1 to activate systemic immunity. The following files are stored here: one MS datasets (include .raw, preprocessed .mgf, and Mascot MS/MS ion search result .dat file) generated in this work, including: “Total identified endogenous peptides observed in SA-treated Arabidopsis leaves on Q Exactive HF (named as supplementaldata2)”
HostingRepository	PRIDE
AnnounceDate	2023-07-20
AnnouncementXML	Submission_2023-07-19_22:05:15.421.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ShengchiHung
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-30 20:53:03	ID requested
⏵ 1	2023-07-19 22:05:15	announced

Dataset with its publication pending

submitter keyword: Systemic acquired resistance,Plant immunity, Protease, Salicylic acid, Peptide cytokine

Yet RanChen
contact affiliation	Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan
contact email	yetran@gate.sinica.edu.tw
lab head
ShengchiHung
contact affiliation	Acdemia Sinica
contact email	kempis710165@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD041898

PRIDE project URI

• PRIDE
  1. PXD041898
     1. Label: PRIDE project
     2. Name: XCP1 cleaves Pathogenesis-related protein 1 into CAPE9 for systemic immunity in Arabidopsis