PXD041861 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Perforin-2 is a pore-forming effector of endocytic escape in cross-presenting dendritic cells |
Description | This study searched for cell-type specific effectors of endocytic escape in dendritic cells and identified a pore-forming protein, perforin-2 (encoded by Mpeg1), as a dedicated effector exclusive to cross-presenting cells. Whole cell proteomics identified differences in the abundance of tryptic and semi-tryptic (cleaved) perforin-2 peptides between control MutuDCs and cells treated with BafA, CpG or with knockout of asparagine endopeptidase (AEP). This revealed how perforin-2 undergoes proteolytic cleavage releasing its pore forming domain into the organellar lumen. |
HostingRepository | PRIDE |
AnnounceDate | 2023-06-27 |
AnnouncementXML | Submission_2023-06-27_02:53:00.337.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | AlexandraDavies |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF; Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-04-27 16:18:08 | ID requested | |
⏵ 1 | 2023-06-27 02:53:00 | announced | |
2 | 2023-11-14 06:54:26 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Dendritic cells, antigen, cross-presentation, perforin-2 |
Contact List
Georg H. H.Borner |
contact affiliation | Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany |
contact email | borner@biochem.mpg.de |
lab head | |
AlexandraDavies |
contact affiliation | University of Manchester |
contact email | alexandra.davies-2@manchester.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD041861 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041861
- Label: PRIDE project
- Name: Perforin-2 is a pore-forming effector of endocytic escape in cross-presenting dendritic cells