PXD041858-1
PXD041858 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Light Activated BioID (LAB): an optically activated proximity labeling system to study protein-protein interactions |
| Description | Proximity labeling with genetically encoded enzymes is widely used to study protein-protein interactions in cells. However, the resolution and accuracy of proximity labeling methods are limited by a lack of control over the enzymatic labeling process. Here, we present a high spatial and temporal resolution technology that can be activated on demand using light, for high accuracy proximity labeling. Our system, called Light Activated BioID (LAB), is generated by fusing the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1. Using live cell imaging, immunofluorescence, western blotting, and mass spectrometry, we show that upon exposure to blue light, CRY2 and CIB1 dimerize, reconstitute the split-TurboID enzyme, and biotinylate proximate proteins. Turning off the light halts the biotinylation reaction. We validate LAB in different cell types and demonstrate that it can identify known binding partners of proteins while reducing background labeling and false positives. |
| HostingRepository | MassIVE |
| AnnounceDate | 2023-10-02 |
| AnnouncementXML | Submission_2023-10-02_13:59:22.211.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | gabriela grigorean |
| SpeciesList | scientific name: Canis lupus familiaris; common name: dog; NCBI TaxID: 9615; scientific name: Arabidopsis thaliana; common name: thale cress; NCBI TaxID: 3702; scientific name: Aequorea victoria; NCBI TaxID: 6100; |
| ModificationList | Oxidation; Acetyl; Deamidated |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-04-27 13:51:57 | ID requested | |
| ⏵ 1 | 2023-10-02 13:59:22 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: TurboID, BioID, E-cadherin, Proximity labeling, Light-activated proximity labeling, Split TurboID |
Contact List
| Sanjeevi Sivasankar | |
|---|---|
| contact affiliation | University of California, Davis |
| contact email | ssivasankar@ucdavis.edu |
| lab head | |
| gabriela grigorean | |
| contact affiliation | ucd |
| contact email | ggrigorean@ucdavis.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000091815/ |
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