PXD041854-1

PXD041854 is an original dataset announced via ProteomeXchange.

Title	Plitidepsin is a broad-spectrum antiviral that transiently inhibits viral 1 protein translation and shapes a protective proteostatic cellular response
Description	Plitidepsin is a safe antiviral for COVID-19 patients that impairs SARS-CoV-2 replication by targeting host factors implicated in protein translation. Here we used deep quantitative proteomics coupled to infectious assays and transmission electron microscopy to dissect its antiviral activity. Plitidepsin inhibited the synthesis of all SARS-CoV-2 proteins, including R1AB required for double membrane vesicle formation needed for viral replication. Yet, less than 14% of the cellular proteome was affected by plitidepsin, which up-regulated translation factors such as eIF4A2 and eIF2S3 and ribosomal proteins associated to protein biosynthesis. At a concentration of 50nM, plitidepsin resulted in a compensatory proteostasis that rescued protein translation. Moreover, plitidepsin inhibited other RNA-dependent and non-integrated DNA viruses from the Flaviviridae, Pneumoviridae, and Herpesviridae families, but failed to block DNA-integrated proviruses from the Retroviridae family. Unraveling the mechanism of action of host-directed therapies like plitidepsin is essential to develop broad-spectrum antivirals ready to deploy when future pandemics begin.
HostingRepository	PRIDE
AnnounceDate	2024-12-19
AnnouncementXML	Submission_2024-12-19_07:45:56.924.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joan Josep Bech-Serra
SpeciesList	scientific name: Chlorocebus; NCBI TaxID: 392815;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-27 08:40:06	ID requested
⏵ 1	2024-12-19 07:45:57	announced

Dataset with its publication pending

submitter keyword: plitidepsin, TMT, Orbitrap Eclipse, SARS-CoV-2, COVID-19

Nuria Izquierdo
contact affiliation	IrsiCaixa AIDS Research Institute, Can Ruti Campus, UAB, Badalona, Spain
contact email	nizquierdo@irsicaixa.es
lab head
Joan Josep Bech-Serra
contact affiliation	Proteomics Unit - Josep Carreras Leukaemia Research Institute
contact email	jbech@carrerasresearch.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD041854

PRIDE project URI

• PRIDE
  1. PXD041854
     1. Label: PRIDE project
     2. Name: Plitidepsin is a broad-spectrum antiviral that transiently inhibits viral 1 protein translation and shapes a protective proteostatic cellular response