PXD041790-1

PXD041790 is an original dataset announced via ProteomeXchange.

Title	Secreted Akkermansia muciniphila threonyl-tRNA synthetase functions to monitor and modulate immune homeostasis
Description	Commensal bacteria are critically involved in the establishment of tolerance against inflammatory challenges, the molecular mechanisms of which are just being uncovered. All kingdoms of life produce aminoacyl-tRNA synthetases (ARSs). Thus far, the non-translational roles of ARSs have largely been reported in eukaryotes. Here, we report that the threonyl-tRNA synthetase (AmTARS) of the gut-associated bacterium Akkermansia muciniphila is secreted and functions to monitor and modulate immune homeostasis. Secreted AmTARS triggers M2 macrophage polarization and orchestrates the production of anti-inflammatory IL-10 via its unique, evolutionary-acquired regions, which mediates specific interactions with TLR2. This interaction activates the MAPK and PI3K/AKT signaling pathways, which converge on CREB, leading to an efficient production of IL-10 and suppression of the central inflammatory mediator NF-κB. AmTARS restores IL-10-positive macrophages, increases IL-10 levels in the serum, and attenuates the pathological effects in colitis mice. Thus, commensal tRNA synthetases can act as intrinsic mediators that maintain homeostasis.
HostingRepository	PRIDE
AnnounceDate	2026-05-20
AnnouncementXML	Submission_2026-05-20_02:29:22.485.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ga Seul Lee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-24 20:16:16	ID requested
⏵ 1	2026-05-20 02:29:23	announced

10.1016/J.CHOM.2023.05.007;

submitter keyword: None

Myung Hee Kim
contact affiliation	Korea Research Institute of Bioscience and Biotechnology (KRIBB)
contact email	mhk8n@kribb.re.kr
lab head
Ga Seul Lee
contact affiliation	Korea Research Institute of Bioscience & Biotechnology (KRIBB)
contact email	rktmf1013@kribb.re.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041790
     1. Label: PRIDE project
     2. Name: Secreted Akkermansia muciniphila threonyl-tRNA synthetase functions to monitor and modulate immune homeostasis