PXD041774-2

PXD041774 is an original dataset announced via ProteomeXchange.

Title	LptM promotes oxidative maturation of the lipopolysaccharide translocon by substrate binding mimicry.
Description	Insertion of lipopolysaccharide (LPS) into the outer membrane (OM) of Gram-negative bacteria is mediated by a druggable OM translocon consisting of a beta-barrel membrane protein, LptD, and a lipoprotein, LptE. The beta-barrel assembly machinery (BAM) assembles LptD together with LptE to form a plug-and-barrel structure. In the enterobacterium Escherichia coli, formation of two native disulfide bonds in LptD controls LPS translocon activation. Here we report the discovery of LptM (formerly YifL), a conserved lipoprotein that assembles together with LptD and LptE at the BAM complex. We demonstrate that LptM stabilizes a conformation of LptD that can efficiently acquire native disulfide bonds and be released as mature LPS translocon by the BAM complex. Inactivation of LptM causes the accumulation of non-natively oxidized LptD, making disulfide bond isomerization by DsbC become essential for viability. Our structural prediction and biochemical analyses indicate that LptM binds to sites in both LptD and LptE that are proposed to coordinate LPS insertion into the OM. These results suggest that, by mimicking LPS binding, LptM facilitates oxidative maturation of LptD, thereby activating the LPS translocon.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:05:11.498.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julien Marcoux
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-24 06:40:34	ID requested
1	2023-10-11 06:24:03	announced
⏵ 2	2023-11-14 09:05:11	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:05:32	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: LptDE, native MS, HDX-MS, membrane protein, translocon

Julien Marcoux
contact affiliation	IPBS-Toulouse BP64182 205 route de Narbonne 31077 Toulouse Cedex04
contact email	julien.marcoux@ipbs.fr
lab head
Julien Marcoux
contact affiliation	IPBS
contact email	julien.marcoux@ipbs.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041774
     1. Label: PRIDE project
     2. Name: LptM promotes oxidative maturation of the lipopolysaccharide translocon by substrate binding mimicry.