PXD041766-3

PXD041766 is an original dataset announced via ProteomeXchange.

Title	Remodeling of the focal adhesion complex by hydrogen-peroxide- induced senescence
Description	Cellular senescence is a phenotype characterized by cessation of cell division, which can be caused by exhaustive replication or environmental stress. It is involved in age-related pathophysiological conditions and affects both the cellular cytoskeleton and the prime cellular mechanosensors, focal adhesion complexes. While the size of focal adhesions increases during senescence, it is unknown if and how this is accompanied by a remodeling of the internal focal adhesion structure. Our study uses metal-induced energy transfer to study the axial dimension of focal adhesion proteins from oxidative-stress-induced senescent cells with nanometer precision, and compares these to unstressed cells. We influenced cytoskeletal tension and the functioning of mechanosensitive ion channels using drugs and studied the combined effect of senescence and drug intervention on the focal adhesion structure. We find that H 2 O 2 induced restructuring of the focal adhesion complex indicates a loss of tension and altered talin complexation. Mass spectroscopy-based proteomics confirmed the differential regulation of several cytoskeletal proteins induced by H 2 O 2 treatment.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:52:18.338.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Meytal Radzinski
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-24 02:49:51	ID requested
1	2023-07-05 21:43:53	announced
2	2023-11-14 09:01:59	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:52:19	announced	2024-10-22: Updated project metadata.

Grandy C, Port F, Radzinski M, Singh K, Erz D, Pfeil J, Reichmann D, Gottschalk KE, Remodeling of the focal adhesion complex by hydrogen-peroxide-induced senescence. Sci Rep, 13(1):9735(2023) [pubmed]

10.1038/s41598-023-36347-2;

submitter keyword: LC-MS, oxidation, senescence, 3T3 cell line

Kay-Eberhard Gottschalk
contact affiliation	Ulm University, Institute of Experimental Physics, Ulm, Baden-Württemberg, 89081, Germany
contact email	kay.gottschalk@uni-ulm.de
lab head
Meytal Radzinski
contact affiliation	Hebrew University of Jerusalem
contact email	meytal.radzinski@mail.huji.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD041766

PRIDE project URI

• PRIDE
  1. PXD041766
     1. Label: PRIDE project
     2. Name: Remodeling of the focal adhesion complex by hydrogen-peroxide- induced senescence