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PXD041711-2

PXD041711 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDistinct lactation-associated macrophages exist in murine mammary tissue and human milk
DescriptionMacrophages are involved in immune defense, organogenesis and tissue homeostasis. They also contribute to the different phases of mammary gland remodeling during development, pregnancy and involution post-lactation. Yet, less is known about the dynamics of mammary gland macrophages in the lactation stage. Here, we describe a macrophage population present during lactation in mice. By multi-parameter flow cytometry and single-cell RNA sequencing we reveal this population as distinct from the two resident macrophage subsets present pregestationally. These lactation-induced macrophages (LiMacs) are predominantly monocyte-derived and expand by proliferation in situ concomitant with nursing. LiMacs develop independently of IL-34 but require CSF-1 signaling and are partly microbiota-dependent. Locally, they reside adjacent to the basal cells of the alveoli and extravasate into the milk. Moreover, we also found several macrophage subsets in human milk, resembling LiMacs. Collectively, these findings reveal the emergence of unique macrophages in the mammary gland and milk during lactation.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_09:01:37.009.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEkaterina Petrova
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-04-21 01:58:12ID requested
12023-06-23 06:03:42announced
22023-11-14 09:01:40announced2023-11-14: Updated project metadata.
32024-10-22 05:50:17announced2024-10-22: Updated project metadata.
Publication List
Cansever D, Petrova E, Krishnarajah S, Mussak C, Welsh CA, Mildenberger W, Mulder K, Kreiner V, Roussel E, Stifter SA, Andreadou M, Zwicky P, Jurado NP, Rehrauer H, Tan G, Liu Z, Bl, é, riot C, Ronchi F, Macpherson AJ, Ginhoux F, Natalucci G, Becher B, Greter M, Lactation-associated macrophages exist in murine mammary tissue and human milk. Nat Immunol, 24(7):1098-1109(2023) [pubmed]
Keyword List
submitter keyword: mammary gland,macrophages, lactation, milk
Contact List
Melanie Greter
contact affiliationMyeloid Cell Immunology Institute of Experimental Immunology University of Zurich Switzerland
contact emailgreter@immunology.uzh.ch
lab head
Ekaterina Petrova
contact affiliationUniversity of Zurich
contact emailpetrova@immunology.uzh.ch
dataset submitter
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Dataset FTP location
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