PXD041711-2

PXD041711 is an original dataset announced via ProteomeXchange.

Title	Distinct lactation-associated macrophages exist in murine mammary tissue and human milk
Description	Macrophages are involved in immune defense, organogenesis and tissue homeostasis. They also contribute to the different phases of mammary gland remodeling during development, pregnancy and involution post-lactation. Yet, less is known about the dynamics of mammary gland macrophages in the lactation stage. Here, we describe a macrophage population present during lactation in mice. By multi-parameter flow cytometry and single-cell RNA sequencing we reveal this population as distinct from the two resident macrophage subsets present pregestationally. These lactation-induced macrophages (LiMacs) are predominantly monocyte-derived and expand by proliferation in situ concomitant with nursing. LiMacs develop independently of IL-34 but require CSF-1 signaling and are partly microbiota-dependent. Locally, they reside adjacent to the basal cells of the alveoli and extravasate into the milk. Moreover, we also found several macrophage subsets in human milk, resembling LiMacs. Collectively, these findings reveal the emergence of unique macrophages in the mammary gland and milk during lactation.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:01:37.009.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ekaterina Petrova
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-21 01:58:12	ID requested
1	2023-06-23 06:03:42	announced
⏵ 2	2023-11-14 09:01:40	announced	2023-11-14: Updated project metadata.
3	2024-10-22 05:50:17	announced	2024-10-22: Updated project metadata.

Cansever D, Petrova E, Krishnarajah S, Mussak C, Welsh CA, Mildenberger W, Mulder K, Kreiner V, Roussel E, Stifter SA, Andreadou M, Zwicky P, Jurado NP, Rehrauer H, Tan G, Liu Z, Bl, é, riot C, Ronchi F, Macpherson AJ, Ginhoux F, Natalucci G, Becher B, Greter M, Lactation-associated macrophages exist in murine mammary tissue and human milk. Nat Immunol, 24(7):1098-1109(2023) [pubmed]

submitter keyword: mammary gland,macrophages, lactation, milk

Melanie Greter
contact affiliation	Myeloid Cell Immunology Institute of Experimental Immunology University of Zurich Switzerland
contact email	greter@immunology.uzh.ch
lab head
Ekaterina Petrova
contact affiliation	University of Zurich
contact email	petrova@immunology.uzh.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD041711

PRIDE project URI

• PRIDE
  1. PXD041711
     1. Label: PRIDE project
     2. Name: Distinct lactation-associated macrophages exist in murine mammary tissue and human milk