PXD041707-1

PXD041707 is an original dataset announced via ProteomeXchange.

Title	A bacterial effector manipulates host lysosomal protease activity-dependent plasticity in cell death modalities to facilitate infection
Description	Crosstalk between cell death programs confers appropriate host anti-infection immune responses, but how pathogens remodel this network to facilitate their infections remain largely unclear. Here, we identify mammalian cell entry 3C (Mce3C) as a key regulator of host cell death from Mycobacterium tuberculosis (Mtb), which causes tuberculosis featured with lung inflammation and necrosis. Mce3C binds host lysosomal protease cathepsin B (CTSB), which acts as a decision-maker of cell death modalities, to inhibit the protease activity of CTSB towards BH3-interacting domain death agonist (BID) and receptor-interacting protein kinase 1 (RIPK1), thus preventing the production of pro-apoptotic truncated BID (tBID) while maintaining the abundance of pro-necroptotic RIPK1. Disrupting the Mce3C-CTSB interaction promotes host apoptosis and suppresses necroptosis with attenuated Mtb survival and mitigated lung inflammation in mice. These findings reveal a unique strategy by which the pathogen manipulates host lysosomal protease activity-dependent plasticity between cell death pathways to promote infection and pathogenicity.
HostingRepository	PRIDE
AnnounceDate	2025-01-27
AnnouncementXML	Submission_2025-01-27_05:24:38.436.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhe Lu
SpeciesList	scientific name: Mycobacterium tuberculosis; NCBI TaxID: NCBITaxon:1773; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-20 21:17:36	ID requested
⏵ 1	2025-01-27 05:24:38	announced

Dataset with its publication pending

submitter keyword: Mycobacteria tuberculosis,secreated protein,Mce3C

Cui Hua Liu
contact affiliation	Institute of Microbiology, Beijing
contact email	lu1457883143@gmail.com
lab head
Zhe Lu
contact affiliation	Institute of Microbiology, Beijing
contact email	lu1457883143@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041707
     1. Label: PRIDE project
     2. Name: A bacterial effector manipulates host lysosomal protease activity-dependent plasticity in cell death modalities to facilitate infection