PXD041688 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The ribosomal P-stalk regulates MHC I antigen presentation |
| Description | MHC I antigen processing and presentation (APP) is a highly regulated process that enables CD8+ T cell immunosurveillance. APP begins with the ribosomal synthesis of a source antigen, yet the role of ribosomes, particularly specialized ribosomes, in antigen presentation is poorly understood. Here, we show that the presence of the “P-stalk” on the ribosome enhances antigen presentation. The addition of the P-stalk to the ribosome is stimulated by cytokines that upregulate APP components, and knockdown of one of the P-stalk proteins (P1) reduces T cell recognition of tumor cells. Mechanistically, we show that P1-containing ribosomes exhibit enhanced translation of MHC class I molecules and accessory APP components. Finally, analysis of patient data reveals that the mRNA expression of the P-stalk proteins positively correlates with CD8+ T cell infiltration, a trend not seen for other ribosomal proteins. In all, we demonstrate that the presence of the P-stalk defines a specialized ribosome population that enhances antigen presentation, something that may be exploited by cancer cells to escape immunosurveillance. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-02 |
| AnnouncementXML | Submission_2025-12-02_08:22:31.172.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Onno Bleijerveld |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-04-20 08:57:45 | ID requested | |
| ⏵ 1 | 2025-12-02 08:22:32 | announced | |
Publication List
| 10.1016/j.cell.2024.09.039; |
| Dopler A, Alkan F, Malka Y, van der Kammen R, Hoefakker K, Taranto D, Kocabay N, Mimpen I, Ramirez C, Malzer E, Isaeva OI, Kerkhoff M, Gangaev A, Silva J, Ramalho S, Hoekman L, Altelaar M, Beijersbergen R, Akkari L, Yewdell JW, Kvistborg P, Faller WJ, P-stalk ribosomes act as master regulators of cytokine-mediated processes. Cell, 187(24):6981-6993.e23(2024) [pubmed] |
Keyword List
| submitter keyword: ribosomal, P1, T cell, CD8,LC-MSMS, P-stalk |
Contact List
| Onno Bleijerveld |
|---|
| contact affiliation | Proteomics Facility, Netherlands Cancer Institute, Amsterdam, Netherlands. |
| contact email | O.bleijerveld@nki.nl |
| lab head | |
| Onno Bleijerveld |
|---|
| contact affiliation | The Netherlands Cancer Institute |
| contact email | o.bleijerveld@nki.nl |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041688
- Label: PRIDE project
- Name: The ribosomal P-stalk regulates MHC I antigen presentation
