PXD041682-3

PXD041682 is an original dataset announced via ProteomeXchange.

Title	Yeast eIF4G homogenic whole cell oxidative stress LC-MSMS
Description	Translation initiation factor 4G (eIF4G) is an integral component of the eIF4F complex which is key to translation initiation for most eukaryotic mRNAs. Many eIF4G isoforms have been described in diverse eukaryotic organisms but we currently have a poor understanding of their functional roles and whether they regulate translation in an mRNA specific manner. The yeast Saccharomyces cerevisiae expresses two eIF4G isoforms, eIF4G1 and eIF4G2, that have previously been considered as functionally redundant with any phenotypic differences arising due to alteration in eIF4G expression levels. Using homogenic strains that express eIF4G1 or eIF4G2 as the sole eIF4G isoforms at comparable expression levels to total eIF4G, we show that eIF4G1 is specifically required to mediate the translational response to oxidative stress. We use quantitative proteomics to show that eIF4G1 promotes oxidative stress-specific proteome changes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:09:51.787.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Declan Creamer
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-20 06:25:18	ID requested
1	2023-10-24 12:31:47	announced
2	2023-11-14 09:09:42	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:09:52	announced	2024-10-22: Updated project metadata.

10.1093/nar/gkad568;

Cunningham J, Sfakianos AP, Kritsiligkou P, Kershaw CJ, Whitmarsh AJ, Hubbard SJ, Ashe MP, Grant CM, Paralogous translation factors target distinct mRNAs to differentially regulate tolerance to oxidative stress in yeast. Nucleic Acids Res, 51(16):8820-8835(2023) [pubmed]

submitter keyword: LC-MSMS,Yeast, S. cerevisiae, oxidative stress, whole cell

Chris Grant
contact affiliation	Faculty of Biology, Medicine and Health, The University of Manchester
contact email	chris.grant@manchester.ac.uk
lab head
Declan Creamer
contact affiliation	Faculty of Biology, Medicine and Health, The University of Manchester
contact email	declan.creamer@manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD041682

PRIDE project URI

• PRIDE
  1. PXD041682
     1. Label: PRIDE project
     2. Name: Yeast eIF4G homogenic whole cell oxidative stress LC-MSMS