PXD041681-2

PXD041681 is an original dataset announced via ProteomeXchange.

Title	Changes in the proteome of platelets from patients with critical progression of COVID-19
Description	Platelets, the smallest cells in human blood known for their role in primary haemostasis, are also able to interact with pathogens and play a crucial role in the immune response. In severe coronavirus disease 2019 (COVID-19) cases, platelets become over-activated, resulting in the release of granules, exacerbating inflammation and contributing to the cytokine storm. Here we utilize label-free shotgun proteomics approach to further elucidate the role of platelets in COVID-19 progression and to identify predictive biomarkers for disease outcome. Platelet proteome of the severely ill COVID-19 patients varied significantly from to healthy controls. Significant changes in the levels of proteins associated with protein folding were detected. In addition, a number of proteins with isomerase activity were found in the patient samples, which appear to exert an influence on platelet activity via non-genomic properties of glucocorticoid receptor (GR) and nuclear factor κ-light-chain-enhancer of activated B cells (NFkB). We also performed a comparison of proteins found exclusively in controls, survivors and non-survivors. CRP, CA-1, SAA2 and SEPPINA3 were found to be biomarker candidates in platelets, showing a significant increase in ICU patients.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:10:34.760.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD041681
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Svitlana Rozanova
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-20 06:15:11	ID requested
1	2023-10-24 12:46:55	announced
⏵ 2	2023-11-14 09:10:35	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:10:00	announced	2024-10-22: Updated project metadata.

10.6019/PXD041681;

Wolny M, Rozanova S, Knabbe C, Pfeiffer K, Barkovits K, Marcus K, Birschmann I, Changes in the Proteome of Platelets from Patients with Critical Progression of COVID-19. Cells, 12(17):(2023) [pubmed]

submitter keyword: proteomics, glucocorticoid re-ceptor, ECMO, inflammation, NFkB, acute-phase proteins, carbonic anhydrase 1, COVID-19,platelets

Prof. Katrin Marcus-Alic
contact affiliation	Medizinisches Proteom-Center, Medical Faculty, Ruhr University Bochum, 44801 Bochum, Germany
contact email	katrin.marcus@ruhr-uni-bochum.de
lab head
Svitlana Rozanova
contact affiliation	Ruhr University Bochum, Medical Faculty, Medizinisches Proteom-Center
contact email	Svitlana.Rozanova@ruhr-uni-bochum.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041681
     1. Label: PRIDE project
     2. Name: Changes in the proteome of platelets from patients with critical progression of COVID-19