PXD041666 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Uncovering the proximal proteome of CTR1 through TurboID-mediated proximity labeling
|
| Description | Protein-protein interactions play a crucial role in driving cellular processes and enabling appropriate physiological responses in organisms. The plant hormone ethylene signaling pathway is complex and regulated by the spatiotemporal regulation of its signaling molecules. Constitutive Triple Response 1 (CTR1), a key negative regulator of the pathway, regulates the function of Ethylene-Insensitive 2 (EIN2), a positive regulator of ethylene signaling, at the endoplasmic reticulum (ER) through phosphorylation. Our recent study revealed that CTR1 can also translocate from the ER to the nucleus in response to ethylene and positively regulate ethylene responses by stabilizing EIN3. To gain further insights into the role of CTR1 in plants, we used TurboID proximity labeling and mass spectrometry to identify the proximal proteomes of CTR1 in Nicotiana benthamiana. The identified proximal proteins include known ethylene signaling components, as well as proteins involved in diverse cellular processes such as carbon metabolism, mRNA metabolism, and organelle biogenesis. Our study demonstrates the feasibility of proximity labeling using the N. benthamiana transient expression system and identifies the potential interactors of CTR1 in vivo, unveiling the potential role of CTR1 in a wide range of cellular processes.
|
| HostingRepository | PRIDE |
| AnnounceDate | 2026-05-13 |
| AnnouncementXML | Submission_2026-05-13_07:32:52.078.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Shouling Xu |
| SpeciesList | scientific name: Nicotiana tabacum (Common tobacco); NCBI TaxID: NEWT:4097; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-04-19 19:21:06 | ID requested | |
| ⏵ 1 | 2026-05-13 07:32:52 | announced | |
Publication List
Keyword List
| submitter keyword: CTR1, Proximity labeling, TurboID,Ethylene, Mass spectrometry |
Contact List
| Shouling Xu |
|---|
| contact affiliation | Carnegie MS facility |
| contact email | sxu@ciw.edu |
| lab head | |
| Shouling Xu |
|---|
| contact affiliation | Carnegie Institution at Stanford |
| contact email | sxu@ciw.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD041666 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041666
- Label: PRIDE project
- Name: Uncovering the proximal proteome of CTR1 through TurboID-mediated proximity labeling
