PXD041655 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A Noelin-organized extracellular network of proteins required for constitutive and context-dependent anchoring of AMPA-receptors |
Description | Information processing and storage in the brain rely on AMPA-receptors (AMPARs) and their context-dependent dynamics in synapses and extra-synaptic sites. We found that distribution and dynamics of AMPARs in the plasma membrane are controlled by Noelins, a three-member family of conserved secreted proteins expressed throughout the brain in a cell type-specific manner. Noelin tetramers tightly assemble with the extracellular domains of AMPARs and interconnect them in a network-like configuration with a variety of secreted and membrane-anchored proteins including Neurexin1, Neuritin1, and Seizure 6-like. Knockout of Noelins1-3 profoundly reduced AMPARs in synapses onto excitatory and inhibitory (inter)neurons, decreased their density and clustering in dendrites and abolished activity-dependent synaptic plasticity. Our results uncover an endogenous mechanism for extracellular anchoring of AMPARs and establish Noelin-organized networks as versatile determinants of constitutive and context-dependent neurotransmission. |
HostingRepository | PRIDE |
AnnounceDate | 2024-03-14 |
AnnouncementXML | Submission_2024-03-13_17:24:56.073.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD041655 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Alexander Haupt |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-04-19 10:14:49 | ID requested | |
⏵ 1 | 2024-03-13 17:24:57 | announced | |
Publication List
10.6019/PXD041655; |
Boudkkazi S, Schwenk J, Nakaya N, Brechet A, Kollewe A, Harada H, Bildl W, Kulik A, Dong L, Sultana A, Zolles G, Schulte U, Tomarev S, Fakler B, A Noelin-organized extracellular network of proteins required for constitutive and context-dependent anchoring of AMPA-receptors. Neuron, 111(16):2544-2556.e9(2023) [pubmed] |
10.1016/j.neuron.2023.07.013; |
Keyword List
submitter keyword: AMPA receptors, Proteomics,Molecular Neuroscience |
Contact List
Prof. Dr. Bernd Fakler |
contact affiliation | Institute of Physiology II, University of Freiburg, Germany |
contact email | bernd.fakler@physiologie.uni-freiburg.de |
lab head | |
Alexander Haupt |
contact affiliation | University of Freiburg, Germany |
contact email | alexander.haupt@physiologie.uni-freiburg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041655
- Label: PRIDE project
- Name: A Noelin-organized extracellular network of proteins required for constitutive and context-dependent anchoring of AMPA-receptors