PXD041634-1

PXD041634 is an original dataset announced via ProteomeXchange.

Title	Comparative Proteomics of Calcitriol-treated high- and no-responding Glioma stem-like cells
Description	Glioblastoma is the most common primary brain cancer and is associated with poor survival and high rates of recurrent diseases. The median survival using standard radio-chemotherapy is 15 months and the 5-year survival rate is below 5%. It is hypothesized that treatment resistance, diffuse infiltration of the brain and disease recurrence is, at least in part, due to cancer cells that can obtain a transient, stem-like phenotype. These so-called glioma stem-like cells (GSCs) display pluripotency, express marker proteins associated with stem-cells and can replenish the tumor after treatment. Recently, we have shown that the hormonally active form of vitamin D3, calcitriol (1α,25(OH)2-vitamin D3), is active in a subset of GSCs and reduces traits of stemness. Here, we have employed a set of 8 GSCs that do not respond to calcitriol-treatment (No-responder) and the 8 strongest responders to calcitriol (High-responder) and compared to proteomes after treatment with 50 nM calcitriol for 48h.
HostingRepository	PRIDE
AnnounceDate	2023-11-04
AnnouncementXML	Submission_2023-11-04_12:42:20.286.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ChristianMünch
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-18 17:16:31	ID requested
⏵ 1	2023-11-04 12:42:20	announced
2	2023-11-14 09:12:34	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:13:38	announced	2024-10-22: Updated project metadata.

submitter keyword: Glioma stem-like cells,Calcitriol, Glioblastoma, vitamin D3, Glioma

ChristianMünch
contact affiliation	Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt am Main, Germany Frankfurt Cancer Institute, Frankfurt am Main, Germany Cardio-Pulmonary Institute, Frankfurt am Main, Germany
contact email	bozkurt@med.uni-frankfurt.de
lab head
ChristianMünch
contact affiliation	Goethe University Frankfurt
contact email	ch.muench@em.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD041634

PRIDE project URI

• PRIDE
  1. PXD041634
     1. Label: PRIDE project
     2. Name: Comparative Proteomics of Calcitriol-treated high- and no-responding Glioma stem-like cells