PXD041622-1

PXD041622 is an original dataset announced via ProteomeXchange.

Title	Identification of co-factors of eIF4E1b protein in mouse ovary.
Description	We have identified eukaryotic translation initiation factor 4E family member 1B (Eif4e1b) as a regulator of maternal RNA translation, whose maternal deletion leads to 2-cell arrest phenotype in mouse embryos. eIF4E1b protein binds mRNA selectively in oocytes and controls their translation in early embryos. eIF4E1b expresses specifically in mouse ovary and we have generated a knock-in mouse line in which HA tag is conjugated to the C terminus of eIF4E1b. We aim to identify eIF4E1b co-factors using mass spectrometry after anit-HA immunoprecipitation (IP) using mouse ovaries.
HostingRepository	PRIDE
AnnounceDate	2023-06-12
AnnouncementXML	Submission_2023-06-12_05:18:01.807.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD041622
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	DavidAnderson
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-18 04:14:46	ID requested
⏵ 1	2023-06-12 05:18:02	announced
2	2023-11-14 09:00:54	announced	2023-11-14: Updated project metadata.
3	2024-10-22 05:47:49	announced	2024-10-22: Updated project metadata.

10.6019/PXD041622;

Yang G, Xin Q, Feng I, Wu D, Dean J, Germ cell-specific eIF4E1b regulates maternal mRNA translation to ensure zygotic genome activation. Genes Dev, 37(9-10):418-431(2023) [pubmed]

submitter keyword: Mouse, Initiation facto, eIF-4E1b, affinity, ovary

JurrienDean
contact affiliation	Laboratory of Cellular and Developmental Biology, NIDDK, NIH, US Government
contact email	jurrien.dean@nih.gov
lab head
DavidAnderson
contact affiliation	NIH/NIDDK
contact email	davida@mail.nih.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041622
     1. Label: PRIDE project
     2. Name: Identification of co-factors of eIF4E1b protein in mouse ovary.