PXD041608-1

PXD041608 is an original dataset announced via ProteomeXchange.

Title	Systematic Identification of Post-Transcriptional Regulatory Modules
Description	In our cells, a limited number of RNA binding proteins (RBPs) are responsible for all aspects of RNA metabolism across the entire transcriptome. To accomplish this, RBPs form regulatory units that act on specific target regulons. However, the landscape of RBP combinatorial interactions remains poorly explored. Here, we performed a systematic annotation of RBP combinatorial interactions via multimodal data integration. We built a large-scale map of RBP protein neighborhoods by generating in vivo proximity-dependent biotinylation datasets of 50 human RBPs. In parallel, we used CRISPR interference with single-cell readout to capture transcriptomic changes upon RBP knockdowns. By combining these physical and functional interaction readouts, along with the atlas of RBP mRNA targets from eCLIP assays, we generated an integrated map of functional RBP interactions. We then used this map to match RBPs to their context-specific functions and validated the predicted functions biochemically for four RBPs. This study highlights the previously underappreciated scale of the inter-RBP interactions, be it genetic or physical, and is a first step towards a more comprehensive understanding of post-transcriptional regulatory processes and their underlying molecular grammar.
HostingRepository	PRIDE
AnnounceDate	2024-08-09
AnnouncementXML	Submission_2024-08-09_05:25:35.159.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vishvak Subramanyam
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-17 21:52:24	ID requested
⏵ 1	2024-08-09 05:25:35	announced
2	2024-10-22 06:53:54	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: K562, RBP

Hani Goodarzi
contact affiliation	Department of Biochemistry
contact email	hani.goodarzi@ucsf.edu
lab head
Vishvak Subramanyam
contact affiliation	UCSF Goodarzi Lab
contact email	vishvak.subramanyam@ucsf.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041608
     1. Label: PRIDE project
     2. Name: Systematic Identification of Post-Transcriptional Regulatory Modules