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PXD041589-1

PXD041589 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHigh efficiency preparation of monodisperse plasma membrane derived extracellular vesicles for therapeutic applications
DescriptionExtracellular vesicles (EVs) are highly interesting for the design of next-generation therapeutics. However, their preparation methods face challenges in standardization, yield, and reproducibility. Here, we describe a highly efficient and reproducible EV preparation method for monodisperse nano plasma membrane vesicles (nPMVs), which yields 10 to 100 times more particles per cell and hour than conventional EV preparation methods. nPMVs are produced by homogenizing giant plasma membrane vesicles following cell membrane blebbing and apoptotic body secretion induced by chemical stressors. nPMVs showed no significant differences compared to native EVs from the same cell line in cryo-TEM analysis, in vitro cellular interactions, and in vivo biodistribution studies in zebrafish larvae. Proteomics and lipidomics, on the other hand, suggested substantial differences consistent with the divergent origin of these two EV types and indicated that nPMVs primarily derive from apoptotic extracellular vesicles. nPMVs may provide an attractive source for developing EV based pharmaceutical therapeutics.
HostingRepositoryMassIVE
AnnounceDate2023-04-26
AnnouncementXMLSubmission_2023-04-26_01:04:34.729.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterThomas Bock
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos; Q Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-04-17 13:45:18ID requested
12023-04-26 01:04:35announced
Publication List
no publication
Keyword List
submitter keyword: Extracellular vesicles (EVs), nano plasma membrane vesicles (nPMVs)
Contact List
Alexander Schmidt
contact affiliationBiozentrum, Universtiy of Basel, 4056 Basel, Switzerland
contact emailalex.schmidt@unibas.ch
lab head
Thomas Bock
contact affiliationUni Basel
contact emailthomas.bock@unibas.ch
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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