PXD041589-1

PXD041589 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	High efficiency preparation of monodisperse plasma membrane derived extracellular vesicles for therapeutic applications
Description	Extracellular vesicles (EVs) are highly interesting for the design of next-generation therapeutics. However, their preparation methods face challenges in standardization, yield, and reproducibility. Here, we describe a highly efficient and reproducible EV preparation method for monodisperse nano plasma membrane vesicles (nPMVs), which yields 10 to 100 times more particles per cell and hour than conventional EV preparation methods. nPMVs are produced by homogenizing giant plasma membrane vesicles following cell membrane blebbing and apoptotic body secretion induced by chemical stressors. nPMVs showed no significant differences compared to native EVs from the same cell line in cryo-TEM analysis, in vitro cellular interactions, and in vivo biodistribution studies in zebrafish larvae. Proteomics and lipidomics, on the other hand, suggested substantial differences consistent with the divergent origin of these two EV types and indicated that nPMVs primarily derive from apoptotic extracellular vesicles. nPMVs may provide an attractive source for developing EV based pharmaceutical therapeutics.
HostingRepository	MassIVE
AnnounceDate	2023-04-26
AnnouncementXML	Submission_2023-04-26_01:04:34.729.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Bock
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos; Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-17 13:45:18	ID requested
⏵ 1	2023-04-26 01:04:35	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Extracellular vesicles (EVs), nano plasma membrane vesicles (nPMVs)

submitter keyword: Extracellular vesicles (EVs), nano plasma membrane vesicles (nPMVs)

Contact List

Alexander Schmidt
contact affiliation	Biozentrum, Universtiy of Basel, 4056 Basel, Switzerland
contact email	alex.schmidt@unibas.ch
lab head
Thomas Bock
contact affiliation	Uni Basel
contact email	thomas.bock@unibas.ch
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000091730/